IL-20R alpha Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56409

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IL-20R alpha Primary Antibodies »

Product Specifications

Immunogen

Amino acids 159-175 of human IL-20 receptor alpha protein were used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in human and chimp, and 82% homologous in mouse and rat.

Specificity

Both the expect ~70 kD band and an unknown ~90 kD band are seen in A375 cell lysate and are blocked by the immunizing peptide. The ~90 kD band alone is seen in A431 cell lysate, and it is also blocked by the immunizing peptide. This antibody was made against a portion of the extracellular domain of IL-20RA. Permeabilization of the cells during testing may affect antibody binding.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IL-20R alpha Antibody - BSA Free

Western Blot: IL-20R alpha Antibody [NB100-56409]

Western Blot: IL-20R alpha Antibody [NB100-56409]

Western Blot: IL-20 R alpha Antibody [NB100-56409] - Analysis of IL-20 R alpha Antibody in A375 cell lysate in the (A) absence and (B) presence of blocking peptide using this antibody at 2 ug/ml.
Flow Cytometry: IL-20R alpha Antibody [NB100-56409]

Flow Cytometry: IL-20R alpha Antibody [NB100-56409]

Flow Cytometry: IL-20 R alpha Antibody [NB100-56409] - Cell-surface flow cytometry analysis of IL-20 R alpha Antibodyin A375 cells using this antibody at 5 ug/10^6 cells. The shaded histogram represents cells only, green represents isotype control, and purple represents anti-IL-20 R alpha Antibody

Applications for IL-20R alpha Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

2-5 ug/10^6 cells

Western Blot

1-3 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.2% gelatin

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-20R alpha

Official Gene Symbol: IL20RA Gene ID: 53832 (Human) Gene Map Locus: 6q22.33-q23.1 (Human) IL20RA, is a subunit of IL20R, a member of Cytokine Class II receptor super family. It consists of a central transmembrane domain and functionally induces IL20 and IL26 signaling in epithelial cells and keratinocytes. It associates with IL-20RB and forms a functional receptor complex for IL20, IL19, and IL24. It also associates with IL-10RB and forms a unique heterodimeric functional receptor complex for IL-26. Upon ligand binding, it induces signaling through activation of STAT3 and STAT1. IL20RA is specifically expressed in skin suggesting a prominent role in growth and proliferation of keratinocytes.

Long Name

Interleukin 20 Receptor alpha

Alternate Names

CRF2-8, IL-20 R alpha, IL-20R1, IL-20Ra, IL20R alpha, IL20RA, zcytor7

Entrez Gene IDs

53832 (Human); 60504 (Mouse)

Gene Symbol

IL20RA

Additional IL-20R alpha Products

Product Documents for IL-20R alpha Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IL-20R alpha Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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