Mouse IL-27 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-06785

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL
View all IL-27 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This kit recognizes Mouse IL-27 in samples. No significant cross-reactivity or interference between Mouse IL-27 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 4.92

Inter-Assay Precision (Precision between assays) 5.21

Recovery for Mouse IL-27 ELISA Kit (Colorimetric)

Recovery

90 - 106 %

Linearity

Mouse IL-27 ELISA Kit (Colorimetric)

Scientific Data Images for Mouse IL-27 ELISA Kit (Colorimetric)

Mouse IL-27 ELISA Kit (Colorimetric)

ELISA: Mouse IL-27 ELISA Kit (Colorimetric) [NBP3-06785] -

ELISA: Mouse IL-27 ELISA Kit (Colorimetric) [NBP3-06785] - Standard Curve Reference

Kit Contents for Mouse IL-27 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-27

IL27 is encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR).

Long Name

Interleukin 27

Alternate Names

IL27

Gene Symbol

IL27

Additional IL-27 Products

Product Documents for Mouse IL-27 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Mouse IL-27 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs

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Associated Pathways

Th1 Differentiation Pathway Th1 Differentiation Pathway Thumbnail