Human IL-28A/IFN-lambda 2 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-06787

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human IL-28A/IFN-lambda 2 in samples. No significant cross-reactivity or interference between Human IL-28A/IFN-lambda 2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 4.88

Inter-Assay Precision (Precision between assays) 5.27

Recovery for Human IL-28A/IFN-lambda 2 ELISA Kit (Colorimetric)

Recovery

88 - 108 %

Linearity

Human IL-28A/IFN-lambda 2 ELISA Kit (Colorimetric)

Scientific Data Images for Human IL-28A/IFN-lambda 2 ELISA Kit (Colorimetric)

Human IL-28A/IFN-lambda 2 ELISA Kit (Colorimetric)

ELISA: Human IL-28A/IFN-lambda 2 ELISA Kit (Colorimetric) [NBP3-06787] -

ELISA: Human IL-28A/IFN-lambda 2 ELISA Kit (Colorimetric) [NBP3-06787] - Standard Curve Reference

Kit Contents for Human IL-28A/IFN-lambda 2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-28A/IFN-lambda 2

IL‑28A/Interferon-lambda 2) is a type III interferon that is upregulated in response to viral infection. It acts primarily on epithelial cells through a receptor complex that contains IL-10 R beta and IL-28 R alpha/IFN-lambda R1. IL-28A promotes the Th1 polarization of dendritic cells in the airway (e.g. production of IL-12 p70 and IFN-gamma) and inhibits Th2 and Th17 mediated inflammation. In the liver, however, the IL-28A induced Th1 cytokine response contributes to inflammation in T cell mediated hepatitis. IL-28A additionally exhibits anti-tumor activity, in part by enhancing IL-12 dependent anti-tumor CTL responses. In contrast, it is upregulated in invasive bladder cancer where it promotes tumor cell migration.

Long Name

Interleukin 28A

Alternate Names

IFN-lambda 2, IL28A

Gene Symbol

IFNL2

Additional IL-28A/IFN-lambda 2 Products

Product Documents for Human IL-28A/IFN-lambda 2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human IL-28A/IFN-lambda 2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

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Associated Pathways

Toll-Like Receptor Signaling Pathways Toll-Like Receptor Signaling Pathway Thumbnail