IL-31RA Antibody - Humanized, IgG2SA, (Research Grade nemolizumab Biosimilar) - Low Endotoxin, Azide and BSA Free

Novus Biologicals | Catalog # NBP3-28357

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG2 expressed in CHO

Format

Low Endotoxin, Azide and BSA Free
View all IL-31RA Primary Antibodies »

Product Specifications

Immunogen

IL-31Ra

Reactivity Notes

Predicted species reactivity: Cynomolgus

Clonality

Monoclonal

Host

Human

Isotype

IgG2

Description

The heavy chain type is huIgG2, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Also known as 'nemolizumab'.

IgG2SA is human IgG2 with S378A mutation.

This product is shipped at ambient temperature. Upon receipt, store immediately at -20C or lower for 12 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Scientific Data Images

IL-31RA Antibody (nemolizumab) - Humanized, IgG2SA

Functional: IL-31RA Antibody (nemolizumab) - Humanized, IgG2SA[NBP3-28357] -

IL-31RA Antibody (nemolizumab) - Humanized, IgG2SA P-STAT3 Assay was evaluated using Du145. The IC50 was approximately 0.1068 ug/ml.
IL-31RA Antibody (nemolizumab) - Humanized, IgG2SA

Functional: IL-31RA Antibody (nemolizumab) - Humanized, IgG2SA[NBP3-28357] -

IL-31RA Antibody (nemolizumab) - Humanized, IgG2SA Activation inhibition was evaluated using Hacat. The max induction fold was approximately 1.86.
IL-31RA Antibody (nemolizumab) - Humanized, IgG2SA

ELISA: IL-31RA Antibody (nemolizumab) - Humanized, IgG2SA[NBP3-28357] -

Immobilized human IL31R His at 2 ug/mL can bind IL-31RA Antibody (nemolizumab) - Humanized, IgG2SA, EC50=0.0153 ug/mL.
IL-31RA Antibody (nemolizumab) - Humanized, IgG2SA

Flow Cytometry: IL-31RA Antibody (nemolizumab) - Humanized, IgG2SA[NBP3-28357] -

Human IL31R HEK293 cells were stained with IL-31RA Antibody (nemolizumab) - Humanized, IgG2SA and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.307 ug/mL.

Applications

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Format

Low Endotoxin, Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-31RA

IL31RA is related to gp130 (IL6ST; MIM 600694), the common receptor subunit for IL6 (MIM 147620)-type cytokines. Oncostatin M receptor (OSMR; MIM 601743) and IL31RA form the heterodimeric receptor through which IL31 (MIM 609509) signals. Expression of IL31RA and OSMR mRNA is induced in activated monocytes, and both mRNAs are constitutively expressed in epithelial cells (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM]

Long Name

Interleukin 31 Receptor A

Alternate Names

CRL3, GLM-R, IL-31 RA, IL31RA

Gene Symbol

IL31RA

UniProt

Q8NI17

Additional IL-31RA Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

IL-6 Family

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Protocols

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FAQs

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