Mouse IL-35 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-06750

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL
View all IL-35 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This kit recognizes Mouse IL-35 in samples. No significant cross-reactivity or interference between Mouse IL-35 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV 5.14

Inter-Assay Precision (Precision between assays) %CV 4.64

Recovery for Mouse IL-35 ELISA Kit (Colorimetric)

Recovery

88 - 110%

Linearity

Mouse IL-35 ELISA Kit (Colorimetric)

Scientific Data Images for Mouse IL-35 ELISA Kit (Colorimetric)

Mouse IL-35 ELISA Kit (Colorimetric)

ELISA: Mouse IL-35 ELISA Kit (Colorimetric) [NBP3-06750] -

ELISA: Mouse IL-35 ELISA Kit (Colorimetric) [NBP3-06750] - Standard Curve Reference

Kit Contents for Mouse IL-35 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-35

IL-35 (Interleukin 35) is an immunosuppressive IL-12 family heterodimeric cytokine that contains a p35 subunit and the EBI3 subunit. It is secreted by Treg and effector T cells and functions through IL-12 R beta 2 and gp130. IL-35 can suppress effector T cell proliferation, inhibit Th17 cell development, and promote the conversion of T cells and B cells into regulatory T and B cells, respectively. It also functions as a regulator of tumor growth.

Long Name

Interleukin 35

Alternate Names

IL35

Gene Symbol

EBI3

Additional IL-35 Products

Product Documents for Mouse IL-35 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mouse IL-35 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Regulatory T Cells (Tregs)

Citations for Mouse IL-35 ELISA Kit (Colorimetric)

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