Amino Acid Sequence: MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-5 as determined by LAL method.

Predicted Molecular Mass

26.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL5 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL-5 Protein

SDS-PAGE: Recombinant Human IL-5 Protein [NBP2-34897]

SDS-Page: Human IL-5 Protein [NBP2-34897]

Formulation, Preparation, and Storage

NBP2-34897

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20mM PB, pH 7.5, 3 % trehalose, 0.1 % Tween-80.
Preservative	Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-5

The IL-5 gene is located on chromosome 5 in humans, in close proximity to the genes encoding IL-3, IL-4, and granulocytemacrophage colony-stimulating factor (GM-CSF), which are often co-expressed in TH2 cells. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunroglobulin secretion. It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL-3 and GM-CSF), this glycoprotein in its active form is a homodimer.

Long Name

Interleukin 5

Alternate Names

BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF

Gene Symbol

IL5

UniProt

P05113

Additional IL-5 Products

Product Documents for Recombinant Human IL-5 Protein

Download Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-5 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.