IL6R Antibody (DM152)

Novus Biologicals | Catalog # NBP3-48288

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # DM152
View all IL6R Primary Antibodies »

Product Specifications

Immunogen

IL6R recombinant protein expressed in mammalian cells (Accession# P08887). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for IL6R Antibody (DM152)

IL6R Antibody (DM152)

Flow Cytometry: IL6R Antibody (DM152) [NBP3-48288]

Flow cytometry analysis with Anti-IL6R (DM152) on HEK293 cells transfected with human IL6R (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).
IL6R Antibody (DM152)

ELISA: IL6R Antibody (DM152) [NBP3-48288]

ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human IL6R protein, His tagged protein PME100109 can bind Rabbit anti-IL6R monoclonal antibody (clone: DM152)in a linear range of 1-100 ng/ml.
IL6R Antibody (DM152)

Flow Cytometry: IL6R Antibody (DM152) [NBP3-48288]

Flow cytometry analysis of antigen binding of rabbit anti-human IL6R mAb(NBP3-48288). (A) NBP3-48288 does not bind to CHO-S cells that do not express IL6R. (B) A clear peak shift of NBP3-48288 was seen compared to the control when incubated with IL6R-expressing THP-1 cells, indicating strong binding of NBP3-48288 to IL6R. Antibodies were incubated at 5 ug/mL.

Applications for IL6R Antibody (DM152)

Application
Recommended Usage

ELISA

1:5000-10000

Flow Cytometry

1:100

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL6R

Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. The protein encoded by this gene is a subunit of the receptor complex for IL6. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Alternate Names

CD126, CD126 antigen, gp80, IL-6 receptor subunit alpha, IL-6R 1, IL-6R subunit alpha, IL-6R-1, IL6RA, IL-6RA, IL-6R-alpha, interleukin 6 receptor, interleukin 6 receptor alpha subunit, interleukin-6 receptor subunit alpha, Membrane glycoprotein 80, MGC104991

Gene Symbol

IL6R

Additional IL6R Products

Product Documents for IL6R Antibody (DM152)

Certificate of Analysis

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Product Specific Notices for IL6R Antibody (DM152)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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