ILF2 Antibody (5P1K2)

Novus Biologicals | Catalog # NBP3-16819

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5P1K2 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ILF2 (Q12905). SALAAIRHARWFEENASQSTVKVLIRLLKDLRIRFPGFEPLTPWILDLLGHYAVMNNPTRQPLALNVAYRRCLQILAAGLFLPGSVGITDPCESGNFRVHT

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ILF2 Antibody (5P1K2)

Western Blot: ILF2 Antibody (5P1K2) [NBP3-16819]

Western Blot: ILF2 Antibody (5P1K2) [NBP3-16819]

Western Blot: ILF2 Antibody (5P1K2) [NBP3-16819] - Western blot analysis of extracts of various cell lines, using ILF2 antibody (NBP3-16819) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: ILF2 Antibody (5P1K2) [NBP3-16819]

Immunohistochemistry-Paraffin: ILF2 Antibody (5P1K2) [NBP3-16819]

Immunohistochemistry-Paraffin: ILF2 Antibody (5P1K2) [NBP3-16819] - Immunohistochemistry of paraffin-embedded human breast cancer using ILF2 Rabbit mAb (NBP3-16819) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for ILF2 Antibody (5P1K2)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ILF2

Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression. [provided by RefSeq]

Alternate Names

interleukin enhancer binding factor 2, 45kD, interleukin enhancer binding factor 2, 45kDa, interleukin enhancer-binding factor 2, MGC8391, NF45PRO3063, Nuclear factor of activated T-cells 45 kDa, nuclear factor of activated T-cells, 45-kDa

Gene Symbol

ILF2

Additional ILF2 Products

Product Documents for ILF2 Antibody (5P1K2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ILF2 Antibody (5P1K2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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