Key Product Details

Species Reactivity

Human, Mouse, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6F1

Format

Azide and BSA Free
View all ILF2 Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human ILF2 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse ILF2 Antibody (OTI6F1) - Azide and BSA Free (NBP1-47811) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ILF2 Antibody (OTI6F1) - Azide and BSA Free

Western Blot: ILF2 Antibody (OTI6F1)Azide and BSA Free [NBP2-71036]

Western Blot: ILF2 Antibody (OTI6F1)Azide and BSA Free [NBP2-71036]

Western Blot: ILF2 Antibody (OTI6F1) - Azide and BSA Free [NBP2-71036] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ILF2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ILF2.
Immunocytochemistry/ Immunofluorescence: ILF2 Antibody (OTI6F1) - Azide and BSA Free [NBP2-71036]

Immunocytochemistry/ Immunofluorescence: ILF2 Antibody (OTI6F1) - Azide and BSA Free [NBP2-71036]

Immunocytochemistry/Immunofluorescence: ILF2 Antibody (OTI6F1) - Azide and BSA Free [NBP2-71036] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ILF2.
Immunohistochemistry: ILF2 Antibody (OTI6F1) - Azide and BSA Free [NBP2-71036]

Immunohistochemistry: ILF2 Antibody (OTI6F1) - Azide and BSA Free [NBP2-71036]

Immunohistochemistry: ILF2 Antibody (OTI6F1) - Azide and BSA Free [NBP2-71036] - Staining of paraffin-embedded Kidney tissue using anti-ILF2 mouse monoclonal antibody.
Western Blot: ILF2 Antibody (OTI6F1)Azide and BSA Free [NBP2-71036]

Western Blot: ILF2 Antibody (OTI6F1)Azide and BSA Free [NBP2-71036]

Western Blot: ILF2 Antibody (OTI6F1) - Azide and BSA Free [NBP2-71036] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ILF2 monoclonal antibody.

Applications for ILF2 Antibody (OTI6F1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ILF2

Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression. [provided by RefSeq]

Alternate Names

interleukin enhancer binding factor 2, 45kD, interleukin enhancer binding factor 2, 45kDa, interleukin enhancer-binding factor 2, MGC8391, NF45PRO3063, Nuclear factor of activated T-cells 45 kDa, nuclear factor of activated T-cells, 45-kDa

Gene Symbol

ILF2

Additional ILF2 Products

Product Documents for ILF2 Antibody (OTI6F1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ILF2 Antibody (OTI6F1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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