ILKAP/PP2C delta Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85117

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ILKAP/PP2C delta Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PLLFDDLPPASSTDSGSGGPLLFDDLPPASSGDSGSLATSISQMVKTEGKGAKRKTSEEEKNGSEELVEKKVCKASSVIFGLKGYVAERKGEREEMQDAHV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ILKAP/PP2C delta Antibody - BSA Free

Western Blot: ILKAP/PP2C delta Antibody [NBP1-85117]

Western Blot: ILKAP/PP2C delta Antibody [NBP1-85117]

Western Blot: ILKAP/PP2C delta Antibody [NBP1-85117] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: ILKAP/PP2C delta Antibody [NBP1-85117]

Immunocytochemistry/ Immunofluorescence: ILKAP/PP2C delta Antibody [NBP1-85117]

Immunocytochemistry/Immunofluorescence: ILKAP/PP2C delta Antibody [NBP1-85117] - Staining of human cell line A-431 shows localization to nucleus.
Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117]

Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117]

Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Western Blot: ILKAP/PP2C delta Antibody [NBP1-85117]

Western Blot: ILKAP/PP2C delta Antibody [NBP1-85117]

Western Blot: ILKAP/PP2C delta Antibody [NBP1-85117] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117]

Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117]

Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117] - Staining of human stomach shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117]

Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117]

Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117] - Staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117]

Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117]

Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117]

Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117]

Immunohistochemistry-Paraffin: ILKAP/PP2C delta Antibody [NBP1-85117] - Staining of human endometrium shows strong nuclear positivity in glandular cells.

Applications for ILKAP/PP2C delta Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ILKAP/PP2C delta

ILKAP is encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway.

Long Name

Integrin-Linked Kinase-Associated Serine/Threonine Phosphatase 2C

Alternate Names

PP2C delta

Gene Symbol

ILKAP

Additional ILKAP/PP2C delta Products

Product Documents for ILKAP/PP2C delta Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ILKAP/PP2C delta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Phosphatases and Regulators

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Protocols

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