Importin-8 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-03821

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 878-1037 of human Importin-8 (NP_006381.2). TRQLVNREDRSKAEKADMEENEEISSDEEETNVTAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALITVQSRDAAWYQLLMAPLSEDQRTALQEVYTLAEHRRTVAEAKKKIEQQGGFTFENKGVLSAFNFGTVPSNN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Importin-8 Antibody - Azide and BSA Free

Western Blot: Importin-8 AntibodyAzide and BSA Free [NBP3-03821]

Western Blot: Importin-8 AntibodyAzide and BSA Free [NBP3-03821]

Western Blot: Importin-8 Antibody [NBP3-03821] - Analysis of extracts of various cell lines, using Importin-8 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Importin-8 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Importin-8 Antibody - Azide and BSA Free [NBP3-03821] -

Immunocytochemistry/ Immunofluorescence: Importin-8 Antibody - Azide and BSA Free [NBP3-03821] - Immunofluorescence analysis of C6 cells using Importin-8 Rabbit pAb (A14679) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: Importin-8 Antibody - Azide and BSA Free [NBP3-03821]

Immunocytochemistry/ Immunofluorescence: Importin-8 Antibody - Azide and BSA Free [NBP3-03821]

Immunocytochemistry/Immunofluorescence: Importin-8 Antibody [NBP3-03821] - Analysis of L929 cells using Importin-8 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: Importin-8 Antibody - Azide and BSA Free [NBP3-03821]

Immunocytochemistry/ Immunofluorescence: Importin-8 Antibody - Azide and BSA Free [NBP3-03821]

Immunocytochemistry/Immunofluorescence: Importin-8 Antibody [NBP3-03821] - Analysis of HeLa cells using Importin-8 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for Importin-8 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Importin-8

The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. This protein binds to the nuclear pore complex and, along with RanGTP and RANBP1, inhibits the GAP stimulation of the Ran GTPase. [provided by RefSeq]

Alternate Names

FLJ21954, FLJ26580, IMP8, importin 8, importin-8, RAN binding protein 8, Ran-binding protein 8, RanBP8, RANBP8imp8

Gene Symbol

IPO8

Additional Importin-8 Products

Product Documents for Importin-8 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Importin-8 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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