Recombinant Influenza A H1N1 Hemagglutinin (A/New York/3571/2009) His Protein

Novus Biologicals | Catalog # NBP1-99041

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Key Product Details

Source

Baculovirus

Tag

His

Applications

SDS-PAGE
View all Influenza A H1N1 Hemagglutinin Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 18-344 of Influenza A-H1N1 (human) Hemagglutinin protein.

Source: Baculovirus

Amino Acid Sequence:
ADLMDTLCIG YHANNSTDTV DTVLEKNVTV THSVNLLEDK HNGKLCKLRG VAPLHLGKCN IAGWILGNPE CESLSTASSW SYIVETSSSD NGTCYPGDFI DYEELREQLS SVSSFERFEI FPKTSSWPNH DSNKGVTAAC PHAGAKSFYK NLIWLVKKGN SYPKLSKSYI NDKGKEVLVL WGIHHPSTSA DQQSLYQNAD AYVFVGSSRY SKKFKPEIAI RPKVRDQEGR MNYYWTLVEP GDKITFEATG NLVVPRYAFA MERNAGSGII ISDTPVHDCN TTCQTPKGAI NTSLPFQNIH PITIGKCPKY VKSTKLRLAT GLRNVPSIQS RSRHHHHHH

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1ug of protein (determined by LAL method)

Predicted Molecular Mass

37.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Specificity

Influenza A H1N1 Hemagglutinin (A/New York/3571/2009)

Protein / Peptide Type

Recombinant Protein

Scientific Data Images

SDS-PAGE: Recombinant Influenza A H1N1 Hemagglutinin (A/New York/3571/2009) His Protein [NBP1-99041]

SDS-PAGE: Recombinant Influenza A H1N1 Hemagglutinin (A/New York/3571/2009) His Protein [NBP1-99041]

SDS-Page: Recombinant Influenza A H1N1 Hemagglutinin (A/New York/3571/2009), His Protein [NBP1-99041] - 15% SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-99041
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Influenza A H1N1 Hemagglutinin

HA1 (hemaggulutinin1) belongs to the influenza viruses hemagglutinin family. Influenza hemagglutinin (HA) or haemagglutinin is a type of hemagglutinin found on the surface of the influenza viruses. It is an antigenic glycoprotein. It is responsible for binding the virus to the cell that is being infected. HA protein has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding of these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane. Recombinant Influenza A virus (A/ New York/3571/2009 (H1N1)) HA1 protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.

Additional Influenza A H1N1 Hemagglutinin Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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