Recombinant Influenza A H5N1 Hemagglutinin (A/Vietnam/HN31242/2007) His Protein

Novus Biologicals | Catalog # NBP1-99028

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Key Product Details

Source

Baculovirus

Tag

His

Applications

SDS-PAGE
View all Influenza A H5N1 Hemagglutinin Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 17-338 of Influenza A (H5N1) Influenza A Hemagglutinin H5

Source: Baculovirus Amino Acid Sequence: ADPMDQICIG YHANNSTEQV DTIMEKNVTV THAQDILEKT HNGKLCDLDG VKPLILRDCS VAGWLLGNPM CDEFINVPEW SYIVEKANPA NDLCYPGNFN DYEELKHLLS RINHFEKIQI IPKSSWSDHE ASSGVSSACP YQGVPSFFRN VVWLIKKNNT YPTIKRSYNN TNQEDLLILW GIHHSNDAAE QTKLYQNPTT YISVGTSTLN QRLVPKIATR SKVNGQSGRM DFFWTILKPN DAINFESNGN FIAPEYAYKI VKKGDSAIMK SEVEYGNCNT KCQTPIGAIN SSMPFHNIHP LTIGECPKYV KSNKLVLATG LRNSPLHHHH HH

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1ug of protein (determined by LAL method)

Predicted Molecular Mass

37.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Specificity

Influenza A H5N1 Hemagglutinin (A/Vietnam/HN31242/2007)

Protein / Peptide Type

Recombinant Protein

Scientific Data Images

SDS-PAGE: Recombinant Influenza A H5N1 Hemagglutinin (A/Vietnam/HN31242/2007) His Protein [NBP1-99028]

SDS-PAGE: Recombinant Influenza A H5N1 Hemagglutinin (A/Vietnam/HN31242/2007) His Protein [NBP1-99028]

SDS-Page: Recombinant Influenza A Virus H5N1 Influenza A H5N1 Hemagglutinin (A/Vietnam/HN31242/2007) His Protein [NBP1-99028] - 15% SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-99028
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Influenza A H5N1 Hemagglutinin

H5N1 is a subtype of the species Influenza A virus of the Influenzavirus A genus of the Orthomyxoviridae family. consists of single-stranded eight-segment negative-sense genomic RNAs, helical viral ribonucleoprotein (RNP) complexes (RNA segments NP, PB2, PB1 and PA), three viral envelope proteins (hemagglutinin [HA], neuraminidase [NA], and M2 ion channel), and a maxtir (M1) protein. Influenza A viruses are further classified into 16 HA (H1-H16) and 9 NA (N1-N9) serotypes based on the antigenic characteristics of HA and NA envelope glycoproteins. It is responsible for binding the virus to the cell that is being infected. HA protein has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding of these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.

Additional Influenza A H5N1 Hemagglutinin Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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FAQs

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  • Q:  I would like to know this Hemaglutinin is produced in E coli. Is the protein without glicosilation. glicosilation is important immunogen, so what is the purpose to produce the protein without glicosilation,Is it also usfull as antigen?

    A: Yest, NBP1-99028 is a recombinant protein produced by E.coli. Therefore, we expect it to be without glycoslyation. Depending on the protein, glycosylation may be important. Some proteins do not require glycoslyation for it to be folded properly and/or be fully functional. E.coli production is more economical compared to using mammlian cell lines. In this case, NBP1-99028 is designed to be used for PAGE applications. As the protein is mainly used in a state that is denatured, it is not required to be structurally intact.

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