INSTA-Blot Rat Tissues
Novus Biologicals | Catalog # NBP2-30112
![SDS-PAGE: INSTA-Blot Rat Tissues [NBP2-30112]](https://resources.rndsystems.com/images/products/INSTA-Blot-Rat-Tissues-SDS-Page-NBP2-30112-img0006.jpg "SDS-PAGE: INSTA-Blot Rat Tissues [NBP2-30112]")
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Product Summary for INSTA-Blot Rat Tissues
Ready-to-use PVDF membrane with 11 rat tissue lysates (20 ug total protein per lane)
INSTA-Blot™is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot™ Rat Tissues NBP2-30112 is a simple and fast solution for screening proteins from various rat tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot™, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
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Product Specifications
Application Notes
Tissues are from Sprague Dawley rats. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).
Scientific Data Images for INSTA-Blot Rat Tissues
SDS-PAGE: INSTA-Blot Rat Tissues [NBP2-30112]
SDS-Page: INSTA-Blot Rat Tissues [NBP2-30112] - Rat Tissues. Approximately 20 ug per lane of different rat tissue lysate are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. TestisFormulation, Preparation, and Storage
Preparation Method
INSTA-Blot™ Rat Tissues NBP2-30112 contains denatured proteins from rat lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by
SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot™ is sold ready-to-use in your western blot protocol.
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at room temperature.
Background: INSTA-Blot Rat Tissues
Alternate Names
INSTA Blot Rat Tissues, INSTA-Blot Rat, INSTABlot Rat Tissues
Additional INSTA-Blot Rat Tissues Products
Product Documents for INSTA-Blot Rat Tissues
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for INSTA-Blot Rat Tissues
The molecular weights for the marker used with this product are: 180, 116, 97, 66, 44, 29, 18, 14, 6.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
Citations for INSTA-Blot Rat Tissues
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Protocols
View specific protocols for INSTA-Blot Rat Tissues (NBP2-30112):
INSTA-Blot Rat Tissues:
Material Safety Data Sheet for Non Hazardous Products
Hazard Information
Chemical Name: Non hazardous products.
Chemical Formula: N/A
CAS Number: N/A
EEC-No: N/A
Hazard Identification
None
First Aid Measures
Eye Contact: None
Skin Contact: None
Inhalation: None
Ingestion: None
Accidental Release Measures
This product either does not contain hazardous constituents or the concentration of all chemical constituents are below the regulatory threshold limits described by Occupational Safety Health Administration Hazard Communication Standard 29 CFR 1910.1200 and the European Directive 91/155/EEC. 88/379/EEC, and 67/546/EEC.
Handling and Storage
Exposure Controls / Personal Protection
Other Precautions: None
Physical and Chemical Properties
Form: N/A
Color: N/A
Odor: N/A
Melting Point: N/A
Boiling Temperature: N/A
Density: N/A
Vapor Pressure: N/A
Solubility in Water: N/A
Flash Point: N/A
Explosion limits: N/A
Ignition Temperature: N/A
Material Safety Data Sheet for Non Hazardous Products
Hazard Information
Chemical Name: Non hazardous products.
Chemical Formula: N/A
CAS Number: N/A
EEC-No: N/A
Hazard Identification
None
First Aid Measures
Eye Contact: None
Skin Contact: None
Inhalation: None
Ingestion: None
Accidental Release Measures
This product either does not contain hazardous constituents or the concentration of all chemical constituents are below the regulatory threshold limits described by Occupational Safety Health Administration Hazard Communication Standard 29 CFR 1910.1200 and the European Directive 91/155/EEC. 88/379/EEC, and 67/546/EEC.
Handling and Storage
Exposure Controls / Personal Protection
Other Precautions: None
Physical and Chemical Properties
Form: N/A
Color: N/A
Odor: N/A
Melting Point: N/A
Boiling Temperature: N/A
Density: N/A
Vapor Pressure: N/A
Solubility in Water: N/A
Flash Point: N/A
Explosion limits: N/A
Ignition Temperature: N/A
FAQs
No product specific FAQs exist for this product.
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