Integrin alpha 4/CD49d Antibody (2B4) [Janelia Fluor® 646]

Name: Integrin alpha 4/CD49d Antibody (2B4) [Janelia Fluor® 646]
Brand: Novus Biologicals
SKU: BBA37J
Price: 669 USD
Availability: InStock

Novus Biologicals | Catalog # BBA37J

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Adhesion Blockade

Label

Janelia Fluor 646

Antibody Source

Monoclonal Mouse IgG₁ Clone # 2B4

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Product Specifications

Immunogen

U937 human histiocytic lymphoma cell line

Specificity

Detects human Integrin alpha 4 subunit (Needham, L.A. et al. (1994) Cell Adhesion and Communication 2:87).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Integrin alpha 4/CD49d Antibody (2B4) [Janelia Fluor® 646]

Application

Recommended Usage

Adhesion Blockade

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Integrin alpha 4/CD49d

VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).

Alternate Names

CD49d, ITGA4, VLA-4

Gene Symbol

ITGA4

Additional Integrin alpha 4/CD49d Products

Product Documents for Integrin alpha 4/CD49d Antibody (2B4) [Janelia Fluor® 646]

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Product Specific Notices for Integrin alpha 4/CD49d Antibody (2B4) [Janelia Fluor® 646]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.