Human Integrin  alpha 4/CD49d Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (11)
Supplemental Products

Human Integrin  alpha 4/CD49d Antibody Summary

Species Reactivity
Detects human Integrin alpha 4 subunit (Needham, L.A. et al. (1994) Cell Adhesion and Communication 2:87).
Monoclonal Mouse IgG1 Clone # 2B4
Protein A or G purified from hybridoma culture supernatant
U937 human histiocytic lymphoma cell line
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Adhesion Blockade
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized recombinant human VCAM-1 (10 μg/mL, 100 µL/well) was maximally inhibited (80 - 100%) by 5 μg/mL of the antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4/CD49d

Integrin alpha 4, also known as CD49d, is a transmembrane protein that forms heterodimers with beta 1 or beta 7 integrins. These complexes contribute to the trafficking of many hematopoietic cell types. Integrins alpha 4 beta 1 (also known as VLA-4) and alpha 4 beta 7 (LPAM-1) are receptors for fibronectin, VCAM and MAdCAM-1.

Entrez Gene IDs
3676 (Human); 16401 (Mouse); 102128433 (Cynomolgus Monkey)
Alternate Names
269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); ITGA4; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA-4

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Citations for Human Integrin  alpha 4/CD49d Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Humanized mouse model of Rasmussen's encephalitis supports the immune-mediated hypothesis
    Authors: H Kebir, L Carmant, F Fontaine, K Béland, CM Bosoi, NT Sanon, JI Alvarez, S Desgent, CL Pittet, D Hébert, MJ Langlois, RM Rébillard, DK Nguyen, C Cieuta-Wal, GL Holmes, HP Goodkin, JR Mytinger, MB Connolly, A Prat, E Haddad
    J. Clin. Invest., 2018;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Select gp120 V2 domain specific antibodies derived from HIV and SIV infection and vaccination inhibit gp120 binding to ?4?7
    Authors: S Lertjuthap, C Cicala, D Van Ryk, M Liu, J Yolitz, D Wei, F Nawaz, A Doyle, B Horowitch, C Park, S Lu, Y Lou, S Wang, R Pan, X Jiang, F Villinger, SN Byrareddy, PJ Santangelo, L Morris, CK Wibmer, K Biris, RD Mason, J Gorman, J Hiatt, E Martinelli, M Roederer, D Fujikawa, G Gorini, G Franchini, A Arakelyan, AA Ansari, K Pattanapan, XP Kong, AS Fauci, J Arthos
    PLoS Pathog., 2018;14(8):e1007278.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Neutralization
  3. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis
    Authors: T Kobori, S Hamasaki, A Kitaura, Y Yamazaki, T Nishinaka, A Niwa, S Nakao, H Wake, S Mori, T Yoshino, M Nishibori, H Takahashi
    Front Immunol, 2018;9(0):334.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Bilirubin Prevents Atherosclerotic Lesion Formation in Low-Density Lipoprotein Receptor-Deficient Mice by Inhibiting Endothelial VCAM-1 and ICAM-1 Signaling
    Authors: ME Vogel, G Idelman, ES Konaniah, SD Zucker
    J Am Heart Assoc, 2017;6(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells.
    Authors: Yoon S, Lee I, Zhang X, Zapata M, Choi Y
    FEBS Open Bio, 2014;4(0):370-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor beta (TGFbeta)-, epidermal growth factor (EGF)-, and nuclear factor kappaB (NFkappaB)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion.
    Authors: Kang I, Yoon D, Braun K, Wight T
    J Biol Chem, 2014;289(22):15393-404.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. The HIV-1 envelope protein gp120 impairs B cell proliferation by inducing TGF-beta1 production and FcRL4 expression.
    Authors: Jelicic K, Cimbro R, Nawaz F, Huang D, Zheng X, Yang J, Lempicki R, Pascuccio M, Van Ryk D, Schwing C, Hiatt J, Okwara N, Wei D, Roby G, David A, Hwang I, Kehrl J, Arthos J, Cicala C, Fauci A
    Nat Immunol, 2013;14(12):1256-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  8. Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin.
    Authors: Ludwig RJ, Hardt K, Hatting M, Bistrian R, Diehl S, Radeke HH, Podda M, Schon MP, Kaufmann R, Henschler R, Pfeilschifter JM, Santoso S, Boehncke WH
    Immunology, 2009;128(2):196-205.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  9. L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
    Authors: Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM
    Atherosclerosis, 2007;192(1):56-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. High pressure promotes monocyte adhesion to the vascular wall.
    Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
    Circ. Res., 2007;100(8):1226-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Rho family small GTPases control migration of hematopoietic progenitor cells into multicellular spheroids of bone marrow stroma cells.
    Authors: Bug G, Rossmanith T, Henschler R, Kunz-Schughart LA, Schroder B, Kampfmann M, Kreutz M, Hoelzer D, Ottmann OG
    J. Leukoc. Biol., 2002;72(4):837-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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