Integrin alpha 8 Antibody (CL7304) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44412

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL7304

Format

Azide and BSA Free
View all Integrin alpha 8 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P53708, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Integrin alpha 8 Antibody (CL7304) - Azide and BSA Free

Integrin alpha 8 Antibody (CL7304) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody [NBP3-44412]

Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody [NBP3-44412]

Analysis in human lung and pancreas tissues using NBP3-44412 antibody. Corresponding Integrin alpha 8 RNA-seq data are presented for the same tissues.
Integrin alpha 8 Antibody (CL7304) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody [NBP3-44412]

Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody [NBP3-44412]

Staining of human endometrium shows strong membranous positivity in smooth muscle cells.
Integrin alpha 8 Antibody (CL7304) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody [NBP3-44412]

Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody [NBP3-44412]

Staining of human lung shows strong membranous positivity in pneumocytes.
Integrin alpha 8 Antibody (CL7304) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody [NBP3-44412]

Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody [NBP3-44412]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Integrin alpha 8 Antibody (CL7304) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody [NBP3-44412]

Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody [NBP3-44412]

Staining of human prostate shows moderate to strong positivity in smooth muscle cells.
Integrin alpha 8 Antibody (CL7304) - Azide and BSA Free Western Blot: Integrin alpha 8 Antibody [NBP3-44412]

Western Blot: Integrin alpha 8 Antibody [NBP3-44412]

Analysis in human cell line HEK 293.

Applications for Integrin alpha 8 Antibody (CL7304) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 8

ITGA8 is a gene that codes for a protein that functions in the genesis of kidneys and other organs by facilitating the recruitment of mesenchymal cells into epithelial structures as well as working as a neuronal receptor for TNC to regulate neurite outgrowth, and is made up of 1063 amino acids, weighs approximately 117 kDa. Studies are being conducted on several diseases and disorders including keratopathy, carcinoma, kidney disease, laryngitis, Parkinson's disease, glomerulonephritis, Alzheimer's disease, melanoma, hepatitis, and neuronitis. ITGA8 has also been shown to have interactions with FN1, ITGB1, ITGA3, NPNT, and VNT in pathways such as the CDK5, Tec kinases signaling, integrin-mediated cell adhesion, signal transduction, and hypertrophic cardiomyopathy pathways.

Alternate Names

ITGA8

Gene Symbol

ITGA8

Additional Integrin alpha 8 Products

Product Documents for Integrin alpha 8 Antibody (CL7304) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Integrin alpha 8 Antibody (CL7304) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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