Integrin beta 4/CD104 Antibody (450-9D) - BSA Free

Novus Biologicals | Catalog # NB100-65599

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 450-9D

Format

BSA Free
View all Integrin beta 4/CD104 Primary Antibodies »

Product Specifications

Immunogen

Purified alpha6 beta4 integrin from A431 cells

Reactivity Notes

Predicted cross-reactivities: Ferret, Mink, Mustelid

Specificity

NB100-65599 recognizes the human beta4 integrin (also known as CD104), a 205kD glycoprotein which associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumour cell lines. NB100-65599 recognizes an extracellular epitope on the CD104 molecule.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Integrin beta 4/CD104 Antibody (450-9D) - BSA Free

Immunohistochemistry-Frozen: Integrin beta 4/CD104 Antibody (450-9D) - BSA Free [NB100-65599]

Immunohistochemistry-Frozen: Integrin beta 4/CD104 Antibody (450-9D) - BSA Free [NB100-65599]

Immunohistochemistry-Frozen: Integrin beta 4/CD104 Antibody (450-9D) [NB100-65599] - Staining of human tonsil cryostat section with Mouse anti human CD104

Applications for Integrin beta 4/CD104 Antibody (450-9D) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:500-1:1000

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Reviewed Applications

Read 1 review rated 4 using NB100-65599 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 4/CD104

Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities.

Alternate Names

CD104, ITGB4

Entrez Gene IDs

3691 (Human)

Gene Symbol

ITGB4

UniProt

P16144

Additional Integrin beta 4/CD104 Products

Product Documents for Integrin beta 4/CD104 Antibody (450-9D) - BSA Free

Certificate of Analysis

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Product Specific Notices for Integrin beta 4/CD104 Antibody (450-9D) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Integrin beta 4/CD104 Antibody (450-9D) - BSA Free

Customer Reviews for Integrin beta 4/CD104 Antibody (450-9D) - BSA Free (1)

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  • Integrin beta 4/CD104 Antibody (450-9D)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human Oral Keratinocyte
    Species: Human
    Verified Customer | Posted 03/14/2018
    Cell monoculture on polymer substrate; 2 hour incubation of primary. Alexa Fluor 594.
    1:1000 dilution
    Integrin beta 4/CD104 Antibody (450-9D) - BSA Free NB100-65599

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