Integrin beta 4/CD104 Antibody (CL6972) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44419

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL6972

Format

Azide and BSA Free
View all Integrin beta 4/CD104 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P16144, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Integrin beta 4/CD104 Antibody (CL6972) - Azide and BSA Free

Integrin beta 4/CD104 Antibody (CL6972) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP3-44419]

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP3-44419]

Analysis in human small intestine and liver tissues using NBP3-44419 antibody. Corresponding Integrin beta 4/CD104 RNA-seq data are presented for the same tissues.
Integrin beta 4/CD104 Antibody (CL6972) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP3-44419]

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP3-44419]

Staining of human liver shows no positivity as expected.
Integrin beta 4/CD104 Antibody (CL6972) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP3-44419]

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP3-44419]

Staining of human placenta shows moderate to strong positivity in basement membrane of trophoblastic cells.
Integrin beta 4/CD104 Antibody (CL6972) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP3-44419]

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP3-44419]

Staining of human prostate shows moderate positivity in basement membrane of glandular cells.
Integrin beta 4/CD104 Antibody (CL6972) - Azide and BSA Free Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP3-44419]

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody [NBP3-44419]

Staining of human small intestine shows moderate positivity in basement membrane of glandular cells.
Integrin beta 4/CD104 Antibody (CL6972) - Azide and BSA Free Western Blot: Integrin beta 4/CD104 Antibody [NBP3-44419]

Western Blot: Integrin beta 4/CD104 Antibody [NBP3-44419]

Analysis in human cell line A-431.

Applications for Integrin beta 4/CD104 Antibody (CL6972) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin beta 4/CD104

Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities.

Alternate Names

CD104, ITGB4

Gene Symbol

ITGB4

Additional Integrin beta 4/CD104 Products

Product Documents for Integrin beta 4/CD104 Antibody (CL6972) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Integrin beta 4/CD104 Antibody (CL6972) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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