Integrin beta 4/CD104 [p Tyr1494] Antibody

Novus Biologicals | Catalog # NBP3-23397

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Integrin beta 4/CD104 Primary Antibodies »

Product Specifications

Immunogen

Phospho-Integrin beta 4/CD104 (Tyr-1494) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1494 of human Integrin beta 4/CD104. This peptide sequence is found in all three Integrin beta 4/CD104 isoforms and is highly conserved in rat and mouse Integrin beta 4/CD104. [UniProt# P16144-2]

Modification

p Tyr1494

Specificity

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-Integrin beta 4/CD104 (Tyr-1494) peptide (without carrier). The antibody detects a 200kDa protein corresponding to the molecular mass of Integrin beta 4/CD104 on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but not in control cells. Similar results were also observed in src-transformed Hct116 cells treated with pervanadate.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Integrin beta 4/CD104 [p Tyr1494] Antibody

Integrin beta 4/CD104 [p Tyr1494] Antibody

Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 [p Tyr1494] Antibody [NBP3-23397] -

Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 [p Tyr1494] Antibody [NBP3-23397] - Immunocytochemical labeling of Integrin beta 4/CD104 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-Integrin beta 4/CD104 (Cytoplasmic region) (left) or rabbit polyclonals anti-Integrin beta 4/CD104 (Tyr-1494) (middle) or anti-Integrin beta 4/CD104 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight(R) 594.
Integrin beta 4/CD104 [p Tyr1494] Antibody

Western Blot: Integrin beta 4/CD104 [p Tyr1494] Antibody [NBP3-23397] -

Western Blot: Integrin beta 4/CD104 [p Tyr1494] Antibody [NBP3-23397] - Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-Integrin beta 4/CD104 (Tyr-1526) (lanes 1 & 2) and anti-Integrin beta 4/CD104 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-Integrin beta 4/CD104 (lanes 5 & 6).

Applications for Integrin beta 4/CD104 [p Tyr1494] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Integrin beta 4/CD104

Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities.

Alternate Names

CD104, ITGB4

Gene Symbol

ITGB4

Additional Integrin beta 4/CD104 Products

Product Documents for Integrin beta 4/CD104 [p Tyr1494] Antibody

Certificate of Analysis

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Product Specific Notices for Integrin beta 4/CD104 [p Tyr1494] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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