IRDye® 680RD, Maleimide
Tocris Bioscience | Catalog # 8877
Key Product Details
Description
Wavelength
Product Description
Key information:IRDye® 680RD, Maleimide is a near-infrared (NIR) fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity).
Application: Fluorescence microscopy.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 680 nm and 694 nm, respectively.
Please see the product protocol for further information and a guide to protein/antibody labeling.
Maleimide technology enables labeling of sulfhydryl groups in the presence of primary and secondary amino groups at physiological or mildly acidic pH. At higher pH there may be competition from the primary and secondary amines.
NOTE: When labeling a peptide or protein with IRDye® 680RD Maleimide, the choice of solvent for the dye is dependent on the stability of the target. Since a small amount of dye is needed to label large (i.e. >45 kDa) proteins, the amount of DMSO in the reaction is negligible and should not affect protein stability. However, since a proportionally larger amount of dye is required to achieve adequate labeling for a small protein (i.e. <45 kDa) or peptide, the level of DMSO in the reaction is more likely to affect the target stability. Dye solubilized in aqueous solutions should be discarded after one use. Dye prepared in DMSO is stable when stored at -20 ºC (for up to two weeks) and protected from light and moisture.
It is recommended to dissolve dye in water or DMSO (10 - 20 mg/mL)
IRDye® is a registered trademark of Li-Cor and is used under license.
Optical Data
| λabs | 672 nm |
| λem | 694 nm |
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Product Specifications for IRDye® 680RD, Maleimide
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Calculators
Background References
References are publications that support the biological activity of the product.
- Sestito Lymphatic-draining nanoparticles deliver Bay K8644 payload to lymphatic vessels and enhance their pumping function. Sci.Adv. 2023 PMID: 36827374
Product Documents for IRDye® 680RD, Maleimide
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IRDye® 680RD, Maleimide
For research use only
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