IRDye® 800CW, NHS ester

Tocris Bioscience | Catalog # 8881

Near-infared dye (reactive group NHS ester)
Tocris Bioscience

Key Product Details

Description

Near-infared dye (reactive group NHS ester)

Wavelength

Excitation = 773 nm, Emission = 792 nm
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Product Description

Key information:

IRDye® 800CW, NHS ester is a near-infrared (NIR) fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines.

Application: Fluorescence microscopy.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 773 nm and 792 nm, respectively.

Please see the protocol for further information on protein/antibody labeling and conjugation.

It is recommended to dissolve this dye in water or DMSO (10 - 20 mg/mL)

NOTE: When labeling a peptide or protein with IRDye® 800CW, NHS ester, the choice of solvent for the dye is dependent on the stability of the target. Since a small amount of dye is needed to label large (i.e. >45 kDa) proteins, the amount of DMSO in the reaction is negligible and should not affect protein stability. However, since a proportionally larger amount of dye is required to achieve adequate labeling for a small protein (i.e.

IRDye® is a registered trademark of Li-Cor and is used under license.

Optical Data

IRDye<sup>®</sup> 800CW, NHS ester Dye Spectra
λabs 773 nm
λem 792 nm
Reactive Group NHS ester

Spectra Viewer

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Product Specifications for IRDye® 800CW, NHS ester

Molecular Weight

1166.20

Formula

C50H54N3Na3O17S4

Storage

Store at -20°C

Purity

≥80% (HPLC)

Chemical Name

Sodium 2-((E)-2-((E)-3-(2-((E)-1-(6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)ethylidene)-2-(4-sulfonatophenoxy)cyclohex-1-en-1-yl)vinyl)-3,3-dimethyl-1-(4-sulfonatobutyl)-3H-indol-1-ium-5-sulfonate

CAS Number

956579-01-4

InChI Key

PBPMREYPAOZRBU-UHFFFAOYSA-K

SMILES

CC1(C)C2=CC(S(=O)(O[Na])=O)=CC=C2[N+](CCCCS([O-])(=O)=O)=C1/C=C/C3=C(OC4=CC=C(S(=O)(O[Na])=O)C=C4)/C(CCC3)=C/C=C5C(C)(C)C(C=C(S(=O)(O[Na])=O)C=C6)=C6N\5CCCCCC(ON7C(CCC7=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product.

Product Documents for IRDye® 800CW, NHS ester

Certificate of Analysis

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Product Specific Notices for IRDye® 800CW, NHS ester

For research use only

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