IRF5 Antibody (6L2B5)
Novus Biologicals | Catalog # NBP3-15343
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6L2B5 expressed in HEK293
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 6-151 of human IRF5 (Q13568). PVAPTPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFCIPWRHATRHGPSQDGDNTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFRLIYDGPRDMPPQPYKIYEVCSNGPAPTDSQPPEDYSFGAGEEEEEEEELQ
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IRF5 Antibody (6L2B5)
Western Blot: IRF5 Antibody (6L2B5) [NBP3-15343]
Western Blot: IRF5 Antibody (6L2B5) [NBP3-15343] - Western blot analysis of extracts of various cell lines, using IRF5 Rabbit mAb (NBP3-15343) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5min.Immunoprecipitation: IRF5 Antibody (6L2B5) [NBP3-15343]
Immunoprecipitation: IRF5 Antibody (6L2B5) [NBP3-15343] - Immunoprecipitation analysis of 600ug extracts of Mouse thymus using 3ug IRF5 antibody (NBP3-15343). Western blot was performed from the immunoprecipitate using IRF5 antibody (NBP3-15343) at a dilition of 1:500.Applications for IRF5 Antibody (6L2B5)
Application
Recommended Usage
Immunoprecipitation
1:100 - 1:500
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IRF5
Long Name
Interferon Regulatory Factor 5
Alternate Names
IBD14, interferon regulatory factor 5, IRF-5, SLEB10
Gene Symbol
IRF5
Additional IRF5 Products
Product Documents for IRF5 Antibody (6L2B5)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IRF5 Antibody (6L2B5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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