ITS Media Supplement (100X)

Insulin-Transferrin-Selenium Media Supplement
Catalog # Availability Size / Price Qty
AR013
Product Details
Procedure
Citations (1)
FAQs
Supplemental Products
Reviews (2)

ITS Media Supplement (100X) Summary

Kit Summary

Supports cell proliferation under low serum conditions.

Key Benefits

  • Contains optimized concentrations of insulin, transferrin, and selenite
  • Chemically-defined media decreases experimental variation
  • High lot-to-lot consistency decreases variation
  • Performance tested on multiple cell lines
 

 

Why Use Pre-optimized and Serum-free Supplements for Cell Culture?

The efficiency of cell proliferation and maintenance of cell health can vary from one experiment to another depending on the media components, the supplements used to drive proliferation, and the quality of the starting cell population.

Although commonly used, serum-containing medium is inherently variable due to the undefined composition and lot-to-lot variability across batches of serum. Troubleshooting media variability can be a costly and time-consuming obstacle that can be minimized through the use of high quality media. Our ITS Media Supplement is a pre-optimized and serum-free media supplement that was designed to help maintain culture media consistency across passages and experiments. The ITS Media Supplement contains optimized concentrations of human insulin, human transferrin, and selenite.

ITS Media Supplement (100x):

  • Contains optimized concentrations of insulin, transferrin, and selenite
  • Chemically-defined media decreases experimental variation
  • High lot-to-lot consistency decreases variation
  • QC-tested on multiple cell lines

 

Kit Contents

5 mL ITS Media Supplement (100x)

Component Amount (µg/mL)
Recombinant Human Insulin 1000
Human Transferrin 500
Selenite 0.67

Stability and Storage

Store in the dark at < -20 °C in a manual defrost freezer. Do not use past the expiration date.

Precautions

This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious reagents, these reagents should be handled as if capable of transmitting infection.

Limitations

  • FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
  • The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
  • This reagent should not be used beyond the expiration date indicated on the label.

 

Data Examples
ITS Media Supplement Supports Proliferation Under Low Serum Conditions
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ITS Media Supplement Supports Proliferation Under Low Serum Conditions. Hela, MCF7, and HepG2 cells were cultured in media supplemented with 10% FBS, low serum (0.1% FBS), or ITS Media Supplement + 0.1% FBS. Cells were trypsinized, harvested, and cell counts were performed. For each respective cell line, cell counts were normalized against standard 10% FBS-containing media (100%).

Product Datasheets

Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, ITS Media Supplement is prepared using the following procedure:

  • Dilute the media into the cell culture media of interest
  • Store completed media at 2 – 8 °C
 

 

Reagents Provided

Reagents provided in the ITS Media Supplement (100X) (Catalog # AR013):

Component Amount (µg/mL)
Recombinant Human Insulin 1000
Human Transferrin 500
Selenite 0.67

 

Stability and Storage

Store in the dark at < -20 °C in a manual defrost freezer. Do not use past the expiration date.

Precautions

This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious reagents, these reagents should be handled as if capable of transmitting infection.

 

Other Supplies Required

Materials

  • Basal media
  • Serological pipettes
  • Pipettes and pipette tips

Equipment

  • 2 °C to 8 °C refrigerator

 

Procedure Overview

Dilute 100-fold into cell culture media of interest before use. The final concentration of serum required with ITS supplementation is cell type and assay dependent. Titrate for individual use.

Citation for ITS Media Supplement (100X)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems
    Authors: V Miceli, M Pampalone, S Vella, AP Carreca, G Amico, PG Conaldi
    Stem Cells Int, 2019;2019(0):7486279.  2019

FAQs

No product specific FAQs exist for this product, however you may

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Reviews for ITS Media Supplement (100X)

Average Rating: 5 (Based on 2 Reviews)

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ITS Media Supplement (100X)
By Anonymous on 06/20/2019

ITS Media Supplement (100X)
By Anonymous on 04/04/2018