ITS Media Supplement (100X)
R&D Systems | Catalog # AR013
Key Product Details
Species
Product Summary for ITS Media Supplement (100X)
Kit Summary
Supports cell proliferation under low serum conditions.
Key Benefits
- Contains optimized concentrations of insulin, transferrin, and selenite
- Chemically-defined media decreases experimental variation
- High lot-to-lot consistency decreases variation
- Performance tested on multiple cell lines
Why Use Pre-optimized and Serum-free Supplements for Cell Culture?
The efficiency of cell proliferation and maintenance of cell health can vary from one experiment to another depending on the media components, the supplements used to drive proliferation, and the quality of the starting cell population.
Although commonly used, serum-containing medium is inherently variable due to the undefined composition and lot-to-lot variability across batches of serum. Troubleshooting media variability can be a costly and time-consuming obstacle that can be minimized through the use of high quality media. Our ITS Media Supplement is a pre-optimized and serum-free media supplement that was designed to help maintain culture media consistency across passages and experiments. The ITS Media Supplement contains optimized concentrations of human insulin, human transferrin, and selenite.
ITS Media Supplement (100x):
- Contains optimized concentrations of insulin, transferrin, and selenite
- Chemically-defined media decreases experimental variation
- High lot-to-lot consistency decreases variation
- QC-tested on multiple cell lines
5 mL ITS Media Supplement (100x)
| Component | Amount (µg/mL) |
|---|---|
| Recombinant Human Insulin | 1000 |
| Human Transferrin | 500 |
| Selenite | 0.67 |
Stability and Storage
Store in the dark at < -20 °C in a manual defrost freezer. Do not use past the expiration date.
Precautions
This product contains human transferrin. This transferrin was purified from donor plasma and tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen.
Limitations
- FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
- The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
- This reagent should not be used beyond the expiration date indicated on the label.
Scientific Data Images for ITS Media Supplement (100X)
ITS Media Supplement Supports Proliferation Under Low Serum Conditions.
ITS Media Supplement Supports Proliferation Under Low Serum Conditions.Hela, MCF7, and HepG2 cells were cultured in media supplemented with 10% FBS, low serum (0.1% FBS), or ITS Media Supplement + 0.1% FBS. Cells were trypsinized, harvested, and cell counts were performed. For each respective cell line, cell counts were normalized against standard 10% FBS-containing media (100%).Formulation, Preparation, and Storage
Shipping
Storage
Product Documents for ITS Media Supplement (100X)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for ITS Media Supplement (100X)
For research use only
Citations for ITS Media Supplement (100X)
Powered by Bioz
Customer Reviews for ITS Media Supplement (100X) (5)
Have you used ITS Media Supplement (100X)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
-
Verified Customer | Posted 01/13/2020
-
Verified Customer | Posted 10/02/2019
-
Verified Customer | Posted 09/30/2019
-
Verified Customer | Posted 06/20/2019
-
Verified Customer | Posted 04/04/2018
There are no reviews that match your criteria.
Protocols
View specific protocols for ITS Media Supplement (100X) (AR013):
Refer to the product datasheet for complete product details.
Briefly, ITS Media Supplement is prepared using the following procedure:
- Dilute the media into the cell culture media of interest
- Store completed media at 2 – 8 °C
Reagents Provided
Reagents provided in the ITS Media Supplement (100X) (Catalog # AR013):
| Component | Amount (µg/mL) |
|---|---|
| Recombinant Human Insulin | 1000 |
| Human Transferrin | 500 |
| Selenite | 0.67 |
Stability and Storage
Store in the dark at < -20 °C in a manual defrost freezer. Do not use past the expiration date.
Precautions
This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious reagents, these reagents should be handled as if capable of transmitting infection.
Other Supplies Required
Materials
- Basal media
- Serological pipettes
- Pipettes and pipette tips
Equipment
- 2 °C to 8 °C refrigerator
Procedure Overview
Dilute 100-fold into cell culture media of interest before use. The final concentration of serum required with ITS supplementation is cell type and assay dependent. Titrate for individual use.