Members of the junctional adhesion molecule (JAM) family are type I transmembrane glycoproteins of the immunoglobulin (Ig) superfamily that are localized in the tight junctions between endothelial or epithelial cells and appear to be involved in leukocyte transmigration. JAM-A, also known as platelet adhesion molecule 1 (PAM-1) and platelet F11 receptor, contains two V-type Ig-like domains, forms homodimers, and interacts with LFA-1. JAM-B (vasculoendothelial or VE-JAM) and JAM-C each contain two Ig domains (one V-type and one C2-type), a cytoplasmic PDZ-binding motif and a PKC phosphorylation site. JAM-C interacts with MAC-1 and facilitates JAM-B interaction with integrin alpha 4 beta 1.
JAM-4/IGSF5 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-98912
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human JAM-4/IGSF5.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for JAM-4/IGSF5 Antibody - Azide and BSA Free
Western Blot: JAM-4/IGSF5 Antibody [NBP2-98912]
Western Blot: JAM-4/IGSF5 Antibody [NBP2-98912] - Anti-JAM-4/IGSF5 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDaApplications for JAM-4/IGSF5 Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: JAM-4/IGSF5
Long Name
Junctional Adhesion Molecule 4
Alternate Names
IGSF5, JAM4, JCAM
Gene Symbol
IGSF5
Additional JAM-4/IGSF5 Products
Product Documents for JAM-4/IGSF5 Antibody - Azide and BSA Free
Certificate of Analysis
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Product Specific Notices for JAM-4/IGSF5 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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