Janelia Fluor® 525, SE
Chemical Name: 3,6-Di-1-(3,3-difluoroazetidinyl)-9-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]xanthylium, inner salt
Biological Activity
Janelia Fluor® 525, SE is a yellow fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Can be multiplexed for two color imaging with Janelia Fluor® 635 SE (Cat. No. 6419). Cell permeable. Excitation maximum = 525 nm; emission maximum = 549 nm; quantum yield = 0.91; Extinction coefficient = 122,000 M-1cm-1; Correction factor A280 = 0.185.Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Optical Data for Janelia Fluor® 525, SE
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 525 nm |
| λem | 549 nm |
| Extinction Coefficient (ε) | 122,000 M-1cm-1 |
| Quantum Yield (φ) | 0.91 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | Confocal imaging, Super resolution microscopy |
| Alternative For | Alexa Fluor® 532, Alexa Fluor® 514, Atto 532, CF514, CF532 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
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Additional Information
Background References
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A general method to fine-tune fluorophores for live-cell and in vivo imaging.
Grimm et al.
Nat. Methods, 2017;14:987
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