Janelia Fluor® 526, NHS ester

Tocris Bioscience | Catalog # 7316

Fluorogenic yellow dye (Reactive group: NHS ester). Application: confocal microscopy, two-color lattice light-sheet microscopy, super resolution microscopy
Tocris Bioscience

Key Product Details

Description

Fluorogenic yellow dye (Reactive group: NHS ester). Application: confocal microscopy, two-color lattice light-sheet microscopy, super resolution microscopy

Alternative Names

JF 526,NHS,JF526

Wavelength

Excitation = 526 nm, Emission = 550 nm
View all Amine-Reactive Janelia Fluor Dyes »

Product Description

Key Information:

Janelia Fluor® 526, NHS ester is a fluorogenic yellow fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines.

Application: Suitable for confocal microscopy, two-color lattice light-sheet microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Cell permeable.

Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Can be multiplexed for two color imaging with Janelia Fluor® 635 NHS ester (Cat. No. 6419). Excitation and emission maxima (λ) are 526 nm and 550 nm, respectively; quantum yield = 0.87; extinction coefficient = 118,000 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.005.

To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.

Please see the protocol for further information on protein/antibody labeling and conjugation.

We also offer custom conjugation antibody services.

HaloTag® is a registered trademark of Promega Corporation, and SNAP-tag® is a registered trademark of New England BioLabs, Inc.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

This product has been recently renamed. The previous name for this product was Janelia Fluor® 526, SE.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Janelia Fluor<sup>®</sup> 526, NHS ester Dye Spectra
Emission Color Yellow
Brightness
λabs 526 nm
λem 550 nm
Extinction Coefficient (ε) 118,000 M-1cm-1
Quantum Yield (φ) 0.87
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Application Confocal Microscopy, Super Resolution Microscopy (SRM), Two-color Lattice Light-sheet Microscopy
Alternative For Alexa Fluor® 532, Alexa Fluor® 514, Atto 532, CF514, CF532

Trademarks and registered trademarks are the property of their respective owners.

Spectra Viewer

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Product Specifications for Janelia Fluor® 526, NHS ester

Molecular Weight

659.50

Formula

C31H19F6N3O7

Storage

Store at -20°C

Chemical Name

2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate

InChI Key

BCNDQOPFHGWJEL-UHFFFAOYSA-N

SMILES

O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC(F)=C(N4CC(F)(F)C4)C=C3O5)=C(C=C/6F)C5=CC6=[N+]7CC(F)(F)C\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 13.19 20

Preparing Stock Solutions for Janelia Fluor® 526, NHS ester

The following data is based on the product molecular weight 659.50.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 7.58 mL 37.91 mL 75.82 mL
1 mM 1.52 mL 7.58 mL 15.16 mL
2 mM 0.76 mL 3.79 mL 7.58 mL
10 mM 0.15 mL 0.76 mL 1.52 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Janelia Fluor® 526, NHS ester

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Janelia Fluor® 526, NHS ester

For research use only

Citations for Janelia Fluor® 526, NHS ester

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