Janelia Fluor 526®, SE

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Janelia Fluor 526®, SE | Amine-Reactive Janelia Fluor Dyes
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Description: Fluorogenic yellow dye; for SMLSM; λabsλem 526/550 nm
Alternative Names: JF 526,NHS, JF526

Chemical Name: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate

Product Details

Biological Activity

Janelia Fluor 526®, SE is a janelia Fluor 526® is a fluorogenic yellow fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super-resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Can be multiplexed for two color imaging with Janelia Fluor® 635 SE (Cat. No. 6419). Cell permeable. Excitation maximum = 526 nm; emission maximum = 550 nm; quantum yield = 0.87; Extinction coefficient = 118,000 M-1cm-1.

To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.

Please see the protocol for further information on protein/antibody labeling and conjugation.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Optical Data for Janelia Fluor 526®, SE

Janelia Fluor 526<sup>®</sup>, SE Dye Spectra
Emission Color Yellow
λabs 526 nm
λem 550 nm
Extinction Coefficient (ε) 118,000 M-1cm-1
Quantum Yield (φ) 0.87
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Application Confocal imaging, Super resolution microscopy, two-color lattice light-sheet microscopy
Alternative For Alexa Fluor® 532, Alexa Fluor® 514, Atto 532, CF514, CF532

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Technical Data

Soluble to 20 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Molarity Calculator


*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).


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