Janelia Fluor 526®, SE
Chemical Name: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate
Biological Activity
Janelia Fluor 526®, SE is a janelia Fluor 526® is a fluorogenic yellow fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super-resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Can be multiplexed for two color imaging with Janelia Fluor® 635 SE (Cat. No. 6419). Cell permeable. Excitation maximum = 526 nm; emission maximum = 550 nm; quantum yield = 0.87; Extinction coefficient = 118,000 M-1cm-1.To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.
Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Optical Data for Janelia Fluor 526®, SE
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 526 nm |
| λem | 550 nm |
| Extinction Coefficient (ε) | 118,000 M-1cm-1 |
| Quantum Yield (φ) | 0.87 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | Confocal imaging, Super resolution microscopy, two-color lattice light-sheet microscopy |
| Alternative For | Alexa Fluor® 532, Alexa Fluor® 514, Atto 532, CF514, CF532 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
Background References
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Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging.
Zheng et al.
ACS Cent.Sci., 2019;5:1602
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