Janelia Fluor® 635, Tetrazine

Tocris Bioscience | Catalog # 8134

Red dye (Reactive group: tetrazine). Application: confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Tocris Bioscience
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Key Product Details

Description

Red dye (Reactive group: tetrazine). Application: confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging

Wavelength

Excitation = 635 nm, Emission = 652 nm

Product Description

Key Information: Janelia Fluor® 635, Tetrazine is a red fluorogenic fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for live-cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Janelia Fluor® 635, Tetrazine is cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 645 nm and 663 nm, respectively.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Janelia Fluor<sup>®</sup> 635, Tetrazine Dye Spectra
Emission Color Red
λabs 635 nm
λem 652 nm
Cell Permeable Yes
Application Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry
Alternative For Alexa Fluor® 633, Atto 633, CF633

Trademarks and registered trademarks are the property of their respective owners.

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Product Specifications for Janelia Fluor® 635, Tetrazine

Molecular Weight

701.81

Formula

C38H33F2N7O3Si

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

4-((4-(1,2,4,5-Tetrazin-3-yl)benzyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate

InChI Key

DVDISOMTYSQEME-UHFFFAOYSA-N

SMILES

C[Si](C1=C/C2=[N+]3CC(C/3)F)(C4=CC(N5CC(C5)F)=CC=C4C(C6=CC(C(NCC7=CC=C(C8=NN=CN=N8)C=C7)=O)=CC=C6C([O-])=O)=C1C=C2)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.02 10

Preparing Stock Solutions for Janelia Fluor® 635, Tetrazine

The following data is based on the product molecular weight 701.81.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 14.25 mL 71.24 mL 142.49 mL
0.5 mM 2.85 mL 14.25 mL 28.50 mL
1 mM 1.42 mL 7.12 mL 14.25 mL
5 mM 0.28 mL 1.42 mL 2.85 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Janelia Fluor® 635, Tetrazine

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Janelia Fluor® 635, Tetrazine

For research use only

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