Janelia Fluor® 635b, NHS ester

Tocris Bioscience | Catalog # 8157

Blinking fluorescent dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM) including SMLM
Tocris Bioscience

Key Product Details

Description

Blinking fluorescent dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM) including SMLM

Wavelength

Excitation = 654 nm, Emission = 671 nm
View all Amine-Reactive Janelia Fluor Dyes »

Product Description

Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to deliver dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.

Key Information: Janelia Fluor® 635b, NHS ester is a spontaneously blinking fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for DNA labelling using amine-terminated oligonucleotides. Also used to label oligonucleotides for super-resolution assays of transposase-accessible chromatin (ATAC-SMLM).

Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).

Properties and Photophysical Data: derived from Janelia Fluor® 635, SE (Cat. No. 6419). Excitation and emission maxima (λ) are 654 nM and 671 nM, respectively.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

Scientific Data Images for Janelia Fluor® 635b, NHS ester

MEF cells labelled with Janelia Fluor 635b, NHS ester

Application of Janelia Fluor® 635b, NHS ester.

SMLM RNA-FISH image of mouse embryo fibroblast cells expressing MS2 in the 3′UTR of the beta-actin gene and labeled with Janelia Fluor® 635b-oligonucleotide from Janelia Fluor® 635b, NHS ester; scale bar: 5 μm. Image kindly provided by Luke D. Lavis, Janelia Research Campus. For more information on this work read Luke D. Lavis et al (2024) A series of spontaneously blinking dyes for super-resolution microscopy. ACS Chem.Biol. bioRxiv. PMID: 38766149
Application of Janelia Fluor® 635b, NHS ester.

Application of Janelia Fluor® 635b, NHS ester.

Representative 3D ATAC–SMLM rendering of accessible chromatin localizations in wild-type mouse embryonic stem (mES) cells using oligonucleotide probes labeled with Janelia Fluor® 635b, NHS ester; the color-coded localization density was calculated with a canopy radius of 250 nm; scale bars: 2 μm. Image kindly provided by Luke D. Lavis, Janelia Research Campus. For more information on this work read Luke D. Lavis et al (2024) A series of spontaneously blinking dyes for super-resolution microscopy. ACS Chem.Biol. bioRxiv. PMID: 38766149

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Janelia Fluor<sup>®</sup> 635b, NHS ester Dye Spectra
λabs 654 nm
λem 671 nm

Spectra Viewer

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Product Specifications for Janelia Fluor® 635b, NHS ester

Molecular Weight

615.71

Formula

C33H31F2N3O5Si

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

2,5-Dioxopyrrolidin-1-yl 3,7-bis(3-fluoroazetidin-1-yl)-5,5-dimethyl-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxylate

InChI Key

VNVYFQFCQSHREC-UHFFFAOYSA-N

SMILES

C[Si]1(C)C2=CC(N3CC(F)C3)=CC=C2C4(OCC5=C4C=C(C(ON6C(CCC6=O)=O)=O)C=C5)C7=CC=C(N8CC(F)C8)C=C71

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product.

Product Documents for Janelia Fluor® 635b, NHS ester

Certificate of Analysis

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Product Specific Notices for Janelia Fluor® 635b, NHS ester

For research use only

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