Janelia Fluor® 646, Azide
Tocris Bioscience | Catalog # 7088
Key Product Details
Description
Alternative Names
Wavelength
Product Description
Key Information:Janelia Fluor® 646, Azide is a red fluorogenic fluorescent dye, supplied with an azide reactive handle for copper-free click chemistry. Suitable for live cell imaging.
Application: Suitable for confocal microscopy and super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Can be multiplexed for two color imaging with Janelia Fluor® 549 SE (Cat. No. 6147). Cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.
To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.
We also offer custom conjugation antibody services.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Brightness | |
| λabs | 646 nm |
| λem | 664 nm |
| Extinction Coefficient (ε) | 152,000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Closest Laser line | 640 nm |
| Reactive Group | Azide |
| Reactivity | Click |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.19 |
| Application | dSTORM, STED, Confocal Microscopy |
| Alternative For | Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5 |
Trademarks and registered trademarks are the property of their respective owners.
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Product Specifications for Janelia Fluor® 646, Azide
Molecular Weight
Formula
Storage
Purity
Chemical Name
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMF | 34.84 | 50 |
Preparing Stock Solutions for Janelia Fluor® 646, Azide
The following data is based on the product molecular weight 696.88.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 2.87 mL | 14.35 mL | 28.70 mL |
| 2.5 mM | 0.57 mL | 2.87 mL | 5.74 mL |
| 5 mM | 0.29 mL | 1.43 mL | 2.87 mL |
| 25 mM | 0.06 mL | 0.29 mL | 0.57 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Zheng Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 2019 PMID: 31572787
- Grimm A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat Methods. 2015 PMID: 25599551
Product Documents for Janelia Fluor® 646, Azide
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Janelia Fluor® 646, Azide
For research use only
Citations for Janelia Fluor® 646, Azide
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Species: MouseAssay Type: In VitroCell Line/Tissue: NeuronsVerified Customer | Posted 09/11/2025Incubate 1:1000 at 37 degrees for 30 to 45 minutes.Works for imaging halo tagged constructs
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