Janelia Fluor® 646, Maleimide

Catalog # Availability Size / Price Qty
6590/2
Janelia Fluor Dyes Small Molecule
1 Image
Description: Red fluorescent dye, supplied with a maleimide reactive group

Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)benzoate

Purity: ≥95%

Product Details
Reviews

Biological Activity

Red fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for confocal fluorescent imaging, super resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells), STED and PAINT imaging. Can be multiplexed for two color imaging with Janelia Fluor® 549 SE (Cat. No. 6147). Cell permeable. Excitation maximum = 646 nm; emission maximum = 664 nm. Quantum yield = 0.54; Max. extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor is 0.19.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

Janelia Fluor® 646, Maleimide Dye Spectra

Spectra charts
Emission color Red
Brightness
λabs 646 nm
λem 664 nm
Extinction coefficient (ε) 152,000 M-1cm-1
Quantum yield (φ) 0.54
Closest Laser line 640 nm
Alternative for Alexa Fluor® 647, Atto 647, Atto 647N*, CF640R, CF647, Cyanine 5

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.


Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data

M.Wt:
618.75
Formula:
C35H34N4O5Si
Solubility:
Soluble to 100 mM in DMSO and to 100 mM in DMF
Purity:
≥95%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Background References

  1. A general method to improve fluorophores for live-cell and single-molecule microscopy.
    Grimm et al.
    Nat.Methods, 2015;12:244

Product Datasheets

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Reconstitution Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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