Janelia Fluor® 646, Tetrazine

Catalog # Availability Size / Price Qty
Janelia Fluor® 646, Tetrazine | CAS No. 2042192-00-5 | Fluorescent Dyes
1 Image
Description: Red dye (Reactive group: tetrazine). Applications: super-resolution microscopy e.g. dSTORM & STED, confocal and live cell imaging
Alternative Names: JF646

Chemical Name: 4-((4-(1,2,4,5-Tetrazin-3-yl)benzyl)carbamoyl)-2-(3-(azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate

Purity: ≥90%

Product Details

Biological Activity

Janelia Fluor® 646, Tetrazine is a red fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for confocal fluorescent imaging, super-resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Can be multiplexed for two-color imaging with Janelia Fluor 549 Tetrazine (Cat. No. 6502). Also suitable for flow cytometry. Cell-permeable. Excitation maximum = 646 nm; emission maximum = 664 nm. Quantum yield = 0.54, Max. extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor is 0.19.

Optical Data for Janelia Fluor® 646, Tetrazine

Janelia Fluor<sup>®</sup> 646, Tetrazine Dye Spectra
Emission Color Red
λabs 646 nm
λem 664 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 647 nm
Reactive Group Tetrazine
Reactivity Copper-free click
Cell Permeable Yes
Correction Factor 260 0.19
Application Confocal imaging, SRM, dSTORM, STED, Flow Cytometry
Alternative For Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data

Soluble to 20 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Complete Your Experiment

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Molarity Calculator


*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).


No product specific FAQs exist for this product, however you may

View all Small Molecule FAQs

Reviews for Janelia Fluor® 646, Tetrazine

There are currently no reviews for this product. Be the first to review Janelia Fluor® 646, Tetrazine and earn rewards!

Have you used Janelia Fluor® 646, Tetrazine?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review
Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.