Janelia Fluor® 646, Tetrazine
Chemical Name: 4-((4-(1,2,4,5-Tetrazin-3-yl)benzyl)carbamoyl)-2-(3-(azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
Purity: ≥90%
Biological Activity
Janelia Fluor® 646, Tetrazine is a red fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for confocal fluorescent imaging, super-resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Can be multiplexed for two-color imaging with Janelia Fluor;® 549 Tetrazine (Cat. No. 6502). Also suitable for flow cytometry. Cell-permeable. Excitation maximum = 646 nm; emission maximum = 664 nm. Quantum yield = 0.54, Max. extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor is 0.19.Optical Data for Janelia Fluor® 646, Tetrazine
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 646 nm |
| λem | 664 nm |
| Extinction Coefficient (ε) | 152,000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Closest Laser line | 647 nm |
| Reactive Group | Tetrazine |
| Reactivity | Copper-free click |
| Cell Permeable | Yes |
| Correction Factor 260 | 0.19 |
| Application | Confocal imaging, SRM, dSTORM, STED, Flow Cytometry |
| Alternative For | Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
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