Janelia Fluor® 646, Tetrazine

Tocris Bioscience | Catalog # 7279

Red dye (Reactive group: tetrazine). Application: flow cytometry, confocal microscopy, super-resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
Tocris Bioscience

Key Product Details

Description

Red dye (Reactive group: tetrazine). Application: flow cytometry, confocal microscopy, super-resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging

Alternative Names

JF646

Wavelength

Excitation = 646 nm, Emission = 664 nm
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Product Description

Key Information:

Janelia Fluor® 646, Tetrazine is a red fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super-resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Can be multiplexed for two-color imaging with Janelia Fluor;&reg 549 Tetrazine (Cat. No. 6502). Cell-permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Janelia Fluor<sup>®</sup> 646, Tetrazine Dye Spectra
Emission Color Red
Brightness
λabs 646 nm
λem 664 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 647 nm
Reactive Group Tetrazine
Reactivity Copper-free click
Cell Permeable Yes
Correction Factor 280 0.19
Application Confocal Microscopy, SRM, dSTORM, STED, Flow Cytometry
Alternative For Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5

Trademarks and registered trademarks are the property of their respective owners.

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Product Specifications for Janelia Fluor® 646, Tetrazine

Molecular Weight

665.83

Formula

C38H35N7O3Si

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

4-((4-(1,2,4,5-Tetrazin-3-yl)benzyl)carbamoyl)-2-(3-(azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate

CAS Number

2042192-00-5

InChI Key

CPWVRCGDSYQFSV-UHFFFAOYSA-N

SMILES

C[Si](C)(C1=C/2)C3=CC(N4CCC4)=CC=C3C(C5=CC(C(NCC6=CC=C(C=C6)C7=NN=CN=N7)=O)=CC=C5C([O-])=O)=C1C=CC2=[N+]8CCC/8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 13.32 20

Preparing Stock Solutions for Janelia Fluor® 646, Tetrazine

The following data is based on the product molecular weight 665.83.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 7.51 mL 37.55 mL 75.09 mL
1 mM 1.50 mL 7.51 mL 15.02 mL
2 mM 0.75 mL 3.75 mL 7.51 mL
10 mM 0.15 mL 0.75 mL 1.50 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Janelia Fluor® 646, Tetrazine

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Janelia Fluor® 646, Tetrazine

For research use only

Citations for Janelia Fluor® 646, Tetrazine

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