Janelia Fluor® 669, Maleimide

Tocris Bioscience | Catalog # 8097

Red dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Tocris Bioscience

Key Product Details

Description

Red dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging

Wavelength

Excitation = 669 nm, Emission = 689 nm
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Product Description

Key information:

Janelia Fluor® 669, Maleimide is a red fluorogenic fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Can be multiplexed for two color imaging with Janelia Fluor® 549 SE (Cat. No. 6147). Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 669 nm and 689 nm, respectively.

Please see the product protocol for further information and a guide to protein/antibody labeling.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer custom conjugation antibody services.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Janelia Fluor<sup>®</sup> 669, Maleimide Dye Spectra
Emission Color Red
λabs 669 nm
λem 689 nm
Reactive Group Maleimide
Reactivity Thiols
Cell Permeable Yes
Application Confocal Microscopy, dSTORM, STED, Flow Cytometry, Live-cell Imaging

Spectra Viewer

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Product Specifications for Janelia Fluor® 669, Maleimide

Molecular Weight

718.82

Formula

C36H33F3N4O5SSi

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)amino)-2-oxoethyl)thio)-3,5,6-trifluorobenzoate

InChI Key

FUZVUVRRODXAGE-UHFFFAOYSA-N

SMILES

C[Si](C1=CC(N2CCC2)=CC=C1C(C3=C(F)C(SCC(NCCN4C(C=CC4=O)=O)=O)=C(F)C(F)=C3C([O-])=O)=C5C=C/6)(C)C5=CC6=[N+]7CCC\7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.19 10

Preparing Stock Solutions for Janelia Fluor® 669, Maleimide

The following data is based on the product molecular weight 718.82.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 13.91 mL 69.56 mL 139.12 mL
0.5 mM 2.78 mL 13.91 mL 27.82 mL
1 mM 1.39 mL 6.96 mL 13.91 mL
5 mM 0.28 mL 1.39 mL 2.78 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Janelia Fluor® 669, Maleimide

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Janelia Fluor® 669, Maleimide

For research use only

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