JNK1/2/3 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-16148

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all JNK Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human JNK1/2/3 (NP_620637.1). MSRSKRDNNFYSVEIGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWSVGCIMGEMVCHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEWKELIYKEVMDLEERTKNG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JNK1/2/3 Antibody - Azide and BSA Free

Western Blot: JNK1/2/3 AntibodyAzide and BSA Free [NBP3-16148]

Western Blot: JNK1/2/3 AntibodyAzide and BSA Free [NBP3-16148]

Western Blot: JNK1/2/3 Antibody [NBP3-16148] - Western blot analysis of extracts of various cell lines, using JNK1/2/3 antibody (NBP3-16148) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunoprecipitation: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148]

Immunoprecipitation: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148]

Immunoprecipitation: JNK1/2/3 Antibody [NBP3-16148] - Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug JNK1/2/3 antibody (NBP3-16148). Western blot was performed from the immunoprecipitate using JNK1/2/3 antibody (NBP3-16148) at a dilition of 1:1000.
JNK1/2/3 Antibody - Azide and BSA Free

Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] -

Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunohistochemistry analysis of paraffin-embedded mouse spleen using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
JNK1/2/3 Antibody - Azide and BSA Free

Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] -

Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunohistochemistry analysis of paraffin-embedded human colon using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
JNK1/2/3 Antibody - Azide and BSA Free

Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] -

Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunohistochemistry analysis of paraffin-embedded mouse brain using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
JNK1/2/3 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] -

Immunocytochemistry/ Immunofluorescence: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunofluorescence analysis of U2OS cells using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for JNK1/2/3 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:200-1:500

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JNK

JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun Nterminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures.

Long Name

C-Jun N-terminal Kinase

Alternate Names

c-Jun N-terminal kinase 1, EC 2.7.11, EC 2.7.11.24, JNK1 alpha protein kinase, JNK1 beta protein kinase, JNK1JNK1A2, JNK-46, JUN N-terminal kinase, MAP kinase 8, MAPK 8, mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated protein kinase 8 isoform JNK1 beta2, PRKM8JNK, protein kinase JNK1, SAPK1JNK21B1/2, Stress-activated protein kinase 1, Stress-activated protein kinase JNK1

Gene Symbol

MAPK8

Additional JNK Products

Product Documents for JNK1/2/3 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for JNK1/2/3 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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