Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 550 and 600 of human KN motif and ankyrin repeat domains 2 (SRC-1 interacting protein) using the numbering given in entry NP_056308.2 (GeneID 25959).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KANK2 Antibody - BSA Free

Western Blot: KANK2 Antibody [NB100-68217]

Western Blot: KANK2 Antibody [NB100-68217]

Western Blot: KANK2 Antibody [NB100-68217] - Detection of Human KANK2/SIP on HeLa whole cell lysate using NB100-68217. KANK2/SIP was also immunoprecipitated by rabbit anti-KANK2/SIP antibody NB100-68218.
Immunohistochemistry: KANK2 Antibody [NB100-68217]

Immunohistochemistry: KANK2 Antibody [NB100-68217]

Immunohistochemistry: KANK2 Antibody [NB100-68217] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- KANK2/SIP used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for KANK2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KANK2

KANK2/SIP was first identified during a genome wide-screen for proteins that interact with the SRC transcriptional coactivator. KANK2/SIP was also identified by domain and phylogenetic analysis to be a member of the KANK ankyrin-repeat domain-containing protein family. KANK related proteins possess a conserved ankyrin-repeat and coiled-coil domain. KANK2, as well as other KANK proteins, appear to play a role in the formation of actin stress fibers.

Alternate Names

ANKRD25, ankyrin repeat domain 25, Ankyrin repeat domain-containing protein 25, DKFZp434N161, FLJ20004, KIAA1518SRC1-interacting protein, kidney ankyrin repeat-containing protein 2, KN motif and ankyrin repeat domain-containing protein 2, KN motif and ankyrin repeat domains 2, Matrix-remodeling-associated protein 3, matrix-remodelling associated 3, MGC119707, MXRA3, SIP, SRC-1 interacting protein, SRC-1-interacting protein

Entrez Gene IDs

25959 (Human)

Gene Symbol

KANK2

Additional KANK2 Products

Product Documents for KANK2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for KANK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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