Kappa Light Chain Antibody (A8B5) [FITC]

Novus Biologicals | Catalog # NBP1-45065

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Goat (Negative), Guinea Pig (Negative), Hamster (Negative), Rabbit (Negative), Sheep (Negative)

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # A8B5
View all Kappa Light Chain Primary Antibodies »

Product Specifications

Immunogen

The antibody A8B5 reacts withIGKC (22.5 kDa) of immunoglobulins.

Specificity

The mouse monoclonal antibody 1D11 recognizes CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Store in the dark. Avoid prolonged exposure to light.

Scientific Data Images for Kappa Light Chain Antibody (A8B5) [FITC]

Flow Cytometry: Kappa Light Chain Antibody (A8B5) [FITC] [NBP1-45065] - Analysis of B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody.
Flow Cytometry: Kappa Light Chain Antibody (A8B5) [FITC] [NBP1-45065] - Surface staining pattern of human peripheral whole blood stained using anti-human Ig kappa light chain (A8B5) FITC antibody (20 ul reagent / 100 ul of peripheral whole blood).
Flow Cytometry: Kappa Light Chain Antibody (A8B5) [FITC] [NBP1-45065] - Multicolor surface staining of human lymphocytes stained using anti-human Ig kappa light chain (A8B5) FITC antibody (20 ul reagent / 100 ul of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 ul reagent / 100 ul of peripheral whole blood).
Flow Cytometry: Kappa Light Chain Antibody (A8B5) [FITC] [NBP1-45065] - Separation of human Ig kappa light chain positive B-lymphocytes (red-filled) from Ig kappa light chain negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Ig kappa light chain (A8B5) FITC antibody (20 ul reagent / 100 ul of peripheral whole blood).

Applications for Kappa Light Chain Antibody (A8B5) [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

Phosphate buffered saline (PBS), pH 7.4

Preservative

15mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Kappa Light Chain

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Alternate Names

HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Gene Symbol

IGKC

Additional Kappa Light Chain Products

Product Documents for Kappa Light Chain Antibody (A8B5) [FITC]

Certificate of Analysis

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Product Specific Notices for Kappa Light Chain Antibody (A8B5) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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