Kappa Light Chain Antibody (A8B5) - BSA Free
Novus Biologicals | Catalog # NBP1-45067
![Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]](https://resources.rndsystems.com/images/products/Kappa-Light-Chain-Antibody-A8B5-Flow-Cytometry-NBP1-45067-img0003.jpg "Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]")
Key Product Details
Species Reactivity
Human, Goat (Negative), Guinea Pig (Negative), Hamster (Negative), Rabbit (Negative), Sheep (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, ELISA, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # A8B5
Format
BSA Free
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Product Specifications
Immunogen
The antibody A8B5 reacts withIGKC (22.5 kDa) of immunoglobulins.
Specificity
The mouse monoclonal antibody 1D11 recognizes CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Kappa Light Chain Antibody (A8B5) - BSA Free
Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]
Flow Cytometry: Kappa Light Chain Antibody (A8B5) [NBP1-45067] - Surface staining of immunoglobulin kappa light chains in human peripheral blood with anti-kappa light chains (A8B5) Pacific BlueTM.![Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]](https://resources.rndsystems.com/images/products/Kappa-Light-Chain-Antibody-A8B5-Flow-Cytometry-NBP1-45067-img0001.jpg "Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]")
Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]
Flow Cytometry: Kappa Light Chain Antibody (A8B5) [NBP1-45067] - B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody.![Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]](https://resources.rndsystems.com/images/products/Kappa-Light-Chain-Antibody-A8B5-Flow-Cytometry-NBP1-45067-img0002.jpg "Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]")
Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]
Flow Cytometry: Kappa Light Chain Antibody (A8B5) [NBP1-45067] - Surface staining of immunoglobulin kappa light chains in human peripheral blood with anti-kappa light chains (A8B5) PE.![Kappa Light Chain Antibody (A8B5) - BSA Free Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]](https://resources.rndsystems.com/images/products/nbp1-45067_mouse-monoclonal-kappa-light-chain-antibody-a8b5-13120241643331.jpg "Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]")
Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]
Separation of human Ig Kappa Light Chain positive lymphocytes (red-filled) from Ig Kappa Light Chain negative lymphocytes (black-dashed) in Analysis (surface staining) of human peripheral whole blood stained using anti-human Ig Kappa Light Chain (A8B5) purified antibody (concentration in sample 4 ug/ml, GAM APC).Applications for Kappa Light Chain Antibody (A8B5) - BSA Free
Application
Recommended Usage
ELISA
1:2000
Flow Cytometry
1-4 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:200
Immunohistochemistry-Paraffin
1:200
Application Notes
This antibody is Cytof ready.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Kappa Light Chain
Alternate Names
HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
Gene Symbol
IGKC
Additional Kappa Light Chain Products
Product Documents for Kappa Light Chain Antibody (A8B5) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Kappa Light Chain Antibody (A8B5) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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