KAT1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-98804

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all KAT1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human KAT1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for KAT1 Antibody - Azide and BSA Free

Western Blot: KAT1 Antibody [NBP2-98804]

Western Blot: KAT1 Antibody [NBP2-98804]

Western Blot: KAT1 Antibody [NBP2-98804] - Anti-KAT1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: U87-MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 49 kDa. Observed band size: 49 kDa
Immunoprecipitation: KAT1 Antibody [NBP2-98804]

Immunoprecipitation: KAT1 Antibody [NBP2-98804]

Immunoprecipitation: KAT1 Antibody [NBP2-98804] - KAT1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate Lane B: 0.5 mg Jurkat Whole Cell Lysate 2 ul anti-KAT1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-KAT1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 49 kDa. Observed band size: 49 kDa

Applications for KAT1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KAT1

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.

Alternate Names

EC 2.3.1.48, histone acetyltransferase 1, histone acetyltransferase type B catalytic subunit, KAT1

Gene Symbol

HAT1

Additional KAT1 Products

Product Documents for KAT1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for KAT1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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