KAT4/TBP Associated Factor 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-29706

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all KAT4/TBP Associated Factor 1 Primary Antibodies »

Product Specifications

Immunogen

between 1800 and 1850

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KAT4/TBP Associated Factor 1 Antibody - BSA Free

KAT4/TBP Associated Factor 1 Antibody

Immunoprecipitation:KAT4/TBP Associated Factor 1 AntibodyNBP3-29706] -

Immunoprecipitation:KAT4/TBP Associated Factor 1 AntibodyNBP3-29706] -Detection of human TAF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TAF1 antibody NBP3-29706 (lot NBP3-29706-2) used for IP at 6 µg per reaction. TAF1 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-29706-1) and rabbit anti-TAF1.. For blotting immunoprecipitated TAF1, NBP3-29706 was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Applications for KAT4/TBP Associated Factor 1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KAT4/TBP Associated Factor 1

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Two transcripts encoding different isoforms have been identified for this gene.

Long Name

Transcription initiation factor TFIID subunit 1

Alternate Names

BA2R, CCG1, CCGS, p250, TAF(II)250, TAF1, TAF2A, TAFII-250, TAFII250

Gene Symbol

TAF1

Additional KAT4/TBP Associated Factor 1 Products

Product Documents for KAT4/TBP Associated Factor 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for KAT4/TBP Associated Factor 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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