KDM6A Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-30213
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
between 700 and 750
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KDM6A Antibody - BSA Free
Immunoprecipitation:KDM6A AntibodyNBP3-30213] -
Immunoprecipitation:KDM6A AntibodyNBP3-30213] -Detection of human UTX by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from LNCaP, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UTX antibody NBP3-30213 (lot NBP3-30213-4) used for IP at 6 µg per reaction. UTX was also immunoprecipitated by a previous lot of this antibody (lot NBP3-30213-3). For blotting immunoprecipitated UTX, NBP3-30213 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.KDM6A AntibodyNBP3-30213] -
Whole cell lysate (50 µg) from LNCaP, HEK293T,Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETNlysis buffer. Antibody: Affinity purified rabbit anti-UTXantibody used for WB at0.04 µg/ml. Detection: Chemiluminescence with anexposure time of 30 seconds.Applications for KDM6A Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 µg/mg lysate
Western Blot
1:10,000 - 1:25,000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Tris-citrate/phosphate buffer, pH 7 to 8
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Lysine (K)-specific Demethylase 6A/KDM6A/UTX
Alternate Names
KABUK2, KDM6A, Lysine (K)specific Demethylase 6A, UTX
Gene Symbol
KDM6A
Additional Lysine (K)-specific Demethylase 6A/KDM6A/UTX Products
Product Documents for KDM6A Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for KDM6A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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