KLF5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88508

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all KLF5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TYTMPSQFLPQQATYFPPSPPSSEPGSPDRQAEMLQNLTPPPSYAATIASKLAIHNPNLPTTLPVNSQNIQPVRYNRRSNPDLEKRRIHYCDYPGCTK

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26212907).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KLF5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: KLF5 Antibody [NBP1-88508]

Immunocytochemistry/ Immunofluorescence: KLF5 Antibody [NBP1-88508]

Immunocytochemistry/Immunofluorescence: KLF5 Antibody [NBP1-88508] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-88508]

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-88508]

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-88508] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-88508]

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-88508]

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-88508] - Staining of human stomach shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-88508]

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-88508]

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-88508] - Staining of human cervix, uterine shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-88508]

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-88508]

Immunohistochemistry-Paraffin: KLF5 Antibody [NBP1-88508] - Staining of human small intestine shows strong nuclear positivity in glandular cells.
KLF5 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: KLF5 Antibody - BSA Free [NBP1-88508]

Chromatin Immunoprecipitation-exo-Seq: KLF5 Antibody - BSA Free [NBP1-88508]

ChIP-Exo-Seq composite graph for Anti-KLF5 (NBP1-88508) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for KLF5 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KLF5

KLF5 encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor.

Long Name

Kruppel-Like Factor 5

Alternate Names

BTEB2

Gene Symbol

KLF5

Additional KLF5 Products

Product Documents for KLF5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for KLF5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KLF5 Antibody - BSA Free

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Protocols

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FAQs

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