KRAS Antibody (3B10-2F2) - Azide and BSA Free

Novus Biologicals | Catalog # H00003845-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B10-2F2

Format

Azide and BSA Free
View all KRAS Primary Antibodies »

Product Specifications

Immunogen

KRAS (AAH13572, 1 a.a. ~ 188 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM

Specificity

KRAS - v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for KRAS Antibody (3B10-2F2) - Azide and BSA Free

Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01]

Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01]

Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western blot analysis of KRAS expression in lysates from human lung cancer cell lines: H23, H358, A549, and H441. Image from verified customer review.
Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01]

Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01]

Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2.

Lane 1: KRAS transfected lysate(21 KDa).
Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: KRAS Antibody (3B10-2F2) [H00003845-M01]

Immunocytochemistry/ Immunofluorescence: KRAS Antibody (3B10-2F2) [H00003845-M01]

Immunocytochemistry/Immunofluorescence: KRAS Antibody (3B10-2F2) [H00003845-M01] - Analysis of monoclonal antibody to KRAS on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01]

Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01]

Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - KRAS monoclonal antibody (M01), clone 3B10-2F2 Analysis of KRAS expression in HeLa.
Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01]

Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01]

KRAS-Antibody-3B10-2F2-Western-Blot-H00003845-M01-img0011.jpg
ELISA: KRAS Antibody (3B10-2F2) [H00003845-M01]

ELISA: KRAS Antibody (3B10-2F2) [H00003845-M01]

ELISA: KRAS Antibody (3B10-2F2) [H00003845-M01] - Detection limit for recombinant GST tagged KRAS is approximately 1ng/ml as a capture antibody.

Applications for KRAS Antibody (3B10-2F2) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for IF and ELISA. IP usage was reported in scientific literature. Customer feedback on frozen sections has been negative.

Reviewed Applications

Read 1 review rated 4 using H00003845-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: KRAS

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Alternate Names

cellular c-Ki-ras2 proto-oncogene, c-Ki-ras, c-K-ras, GTPase KRas, KI-RAS, Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog, K-Ras 2, K-ras p21 protein, KRAS1, K-RAS2A, K-RAS2B, KRAS2NS3, K-RAS4A, K-RAS4B, NS, oncogene KRAS2, PR310 c-K-ras oncogene, RASK2c-Kirsten-ras protein, transforming protein p21, v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Entrez Gene IDs

3845 (Human)

Gene Symbol

KRAS

OMIM

109800 (Human)

UniProt

AAH13572 (Human)

Additional KRAS Products

Product Documents for KRAS Antibody (3B10-2F2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for KRAS Antibody (3B10-2F2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KRAS Antibody (3B10-2F2) - Azide and BSA Free

Customer Reviews for KRAS Antibody (3B10-2F2) - Azide and BSA Free (1)

4 out of 5
1 Customer Rating
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Customer Images

Showing  1 - 1 of 1 review Showing All
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  • Name: Anon ymous
    Application: Western Blot
    Sample Tested: Whole cell lysate from the following human lung cancer lines: H23, H358, A549, H441.
    Species: Human
    Verified Customer | Posted 03/14/2014
    Western blot analysis of KRAS expression in lysates from human lung cancer cell lines: H23, H358, A549, and H441.
    KRAS Antibody (3B10-2F2) - Azide and BSA Free H00003845-M01

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Protocols

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FAQs

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