Kyoto Probe-1

Catalog # Availability Size / Price Qty
7419/100U
Kyoto Probe-1 | CAS No. 2088021-81-0 | Compounds for ESC/iPSC Proliferation and Renewal
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Description: Fluorescent probe that selectively identifies undifferentiated iPS/ES cells
Alternative Names: KP1

Chemical Name: 9-(4-Fluorophenyl)-3-imino-3H-xanthen-6-amine trifluoroacetate

Purity: ≥95%

Product Details
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Biological Activity

Kyoto Probe-1 distinguishes between undifferentiated iPS/ES cells and differentiated cells, so may be used for monitoring pluripotency during hESC or iPSC maintenance. Selectivity of labeling is due to ATP-binding cassette transporters ABCB1 and ABCG2 - these mediate efflux of Kyoto Probe-1 and are expressed in differentiated cells, but are repressed in hiPSC and hESC. Kyoto Probe-1 distinguishes between SSEA-4-positive hESCs and human early hematopoietic cells expressing CD45, CD235, CD41a, or CD43 in flow cytometry experiments, making it a valuable tool for studying early hematopoiesis. The product also distinguishes between hiPSCs and hiPSC-derived cardiomyocytes. Note that Kyoto Probe-1 does not differentiate between hiPSCs and differentiated human neuronal stem cells.

Kyoto Probe-1 localizes to the mitochondria in undifferentiated iPS/ES cells only. Suitable in flow cytometry and live cell imaging.

Excitation maximum = 515 nm; emission maximum = 529 nm; Quantum yield = 0.45

Optical Data for Kyoto Probe-1

Kyoto Probe-1 Dye Spectra
Emission Color Green
λabs 515 nm
λem 529 nm
Quantum Yield (φ) 0.45
Cell Permeable Yes

Technical Data

M.Wt:
418.35
Formula:
C19H13FN2O.CF3CO2H
Solubility:
Soluble to 100 mM in DMSO
Purity:
≥95%
Storage:
Store at -20°C
CAS No:
2088021-81-0

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from New York University

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