Kyoto Probe-1
Tocris Bioscience | Catalog # 7419
Key Product Details
Description
Alternative Names
Wavelength
Product Description
Kyoto Probe-1 distinguishes between undifferentiated iPS/ES cells and differentiated cells, so may be used for monitoring pluripotency during hESC or iPSC maintenance. Selectivity of labeling is due to ATP-binding cassette transporters ABCB1 and ABCG2 - these mediate efflux of Kyoto Probe-1 and are expressed in differentiated cells, but are repressed in hiPSC and hESC. Kyoto Probe-1 distinguishes between SSEA-4-positive hESCs and human early hematopoietic cells expressing CD45, CD235, CD41a, or CD43 in flow cytometry experiments, making it a valuable tool for studying early hematopoiesis. The product also distinguishes between hiPSCs and hiPSC-derived cardiomyocytes. Note that Kyoto Probe-1 does not differentiate between hiPSCs and differentiated human neuronal stem cells.Kyoto Probe-1 localizes to the mitochondria in undifferentiated iPS/ES cells only. Suitable in flow cytometry and live cell imaging.
Excitation maximum = 515 nm; emission maximum = 529 nm; Quantum yield = 0.45
Licensing Information
Sold under license from New York UniversityOptical Data
| Emission Color | Green |
| λabs | 515 nm |
| λem | 529 nm |
| Quantum Yield (φ) | 0.45 |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Live-cell Imaging |
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Product Specifications for Kyoto Probe-1
Molecular Weight
Formula
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Purity
Chemical Name
CAS Number
InChI Key
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The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 41.83 | 100 |
Preparing Stock Solutions for Kyoto Probe-1
The following data is based on the product molecular weight 418.35.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.39 mL | 11.95 mL | 23.90 mL |
| 5 mM | 0.48 mL | 2.39 mL | 4.78 mL |
| 10 mM | 0.24 mL | 1.20 mL | 2.39 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.48 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Hirata A chemical probe that labels human pluripotent stem cells. Cell Rep. 2014 PMID: 24613351
- Miyagi-Shiohira Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells. Sci.Rep. 2020 PMID: 33093580
- Mao A synthetic hybrid molecule for the selective removal of human pluripotent stem cells from cell mixtures. Angew.Chem.Int.Ed.Engl. 2017 PMID: 28067441
Product Documents for Kyoto Probe-1
Certificate of Analysis
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Product Specific Notices for Kyoto Probe-1
For research use only
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