L-Asparaginase Antibody

Novus Biologicals | Catalog # NBP3-50125

Novus Biologicals
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Key Product Details

Species Reactivity

E. coli

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
View all L-Asparaginase Primary Antibodies »

Product Specifications

Immunogen

Asparaginase [Escherichia coli]

Clonality

Polyclonal

Host

Rabbit

Applications for L-Asparaginase Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody has been tested by western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Delipidation and Defibrination

Reconstitution

Reconstitute with 2.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: L-Asparaginase

Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.

Alternate Names

AnsA, AnsB, Asparaginase, Colaspase, Cytoplasmic asparaginase I, L ASNase I, L ASNase II, L asparaginase 1, L asparaginase 2, L asparaginase I, L asparaginase II, L asparaginase II precursor, L asparagine amidohydrolase I, L asparagine amidohydrolase II

Additional L-Asparaginase Products

Product Documents for L-Asparaginase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for L-Asparaginase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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