L-DOPA Antibody

Novus Biologicals | Catalog # NBP3-48640

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

ELISA, Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all L-DOPA Primary Antibodies »

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to Levodopa (L-DOPA)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for L-DOPA Antibody

L-DOPA Antibody

Dot Blot: L-DOPA Antibody [NBP3-48640] -

Dot blot analysis of 100 ng BSA-conjugated levodopa, using levodopa antibody (NBP3-48640) at 1:4000, 1:8000, and 1:16000.
L-DOPA Antibody

ELISA: L-DOPA Antibody [NBP3-48640] -

ELISA plate is coated with 100 ul of levodopa-carrier protein conjugate at 10 ug/mL. The coated ligand is detected with levodopa antibody (NBP3-48640) at concentrations ranging from 360 ng/mL down to 1.3 ng/mL. Rabbit IgG antibody (HRP) is diluted at 1:2,000 and used to detect the primary antibody.

Applications for L-DOPA Antibody

Application
Recommended Usage

ELISA

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: L-DOPA

L Dopa is an intermediate in dopamine biosynthesis. Clinically, L Dopa is used in the management of Parkinson's disease. It is used as a prodrug to increase dopamine levels since it is able to cross the blood-brain barrier whereas dopamine itself cannot. Once L Dopa has entered the central nervous system (CNS), it is metabolised to dopamine by aromatic L amino acid decarboxylase. This also occurs in the peripheral tissues, causing adverse effects and decreasing the available dopamine to the CNS, so it is standard practice to co administer a peripheral DOPA decarboxylase inhibitor and often a catechol-O-methyl transferase (COMT) inhibitor. The antiserum was tested using the free floating PAP technique on rat dopaminergic areas. The anti-conjugated L-DOPA antibodies gave a good staining between 1/2000-1/5000 dilution in these areas.

Alternate Names

3 4 dihydroxy L phenylalanine, L dihydroxyphenylalanine, Levodopa

Additional L-DOPA Products

Product Documents for L-DOPA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for L-DOPA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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