Chemical Name: (2R,3S,4R)-3-Hydroxy-2-[(1S)-1-hydroxy-2-methylpropyl]-4-methyl-5-oxo-2-pyrrolidinecarboxy-N-acetyl-L-cysteine thioester
Biological ActivityLactacystin is a cell-permeable, potent and selective proteasome inhibitor. A Streptomyces metabolite that is thought to bind irreversibly to the active site N-terminal threonine residue of the catalytic β-subunit of the 20S proteasome, thereby inhibiting its chymotrypsin and trypsin-like activities. Induces neurite outgrowth in Neuro 2a neuroblastoma cells and has been reported to induce apoptosis in human monoblast U937 cells. Also inhibits NF-κB activation (IC50 = 10 μM).
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Lactacystin, a novel microbial metabolite, induces neuritogenesis of neuroblastoma cells.
Omura et al.
Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin.
Fenteany et al.
Inhibition of NF-κB activity induces apoptosis in murine hepatocytes.
Bellas et al.
Citations for Lactacystin
The citations listed below are publications that use Tocris products. Selected citations for Lactacystin include:
6 Citations: Showing 1 - 6
Evaluation of Machine Learning Classifiers to Predict Compound Mechanism of Action When Transferred across Distinct Cell Lines.
Authors: Warchal Et al.
SLAS Discov 2019;24:224
The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology.
Authors: Saldate Et al.
J Biol Chem 2018;293:2232
FBG1 Is the Final Arbitrator of A1AT-Z Degradation.
Authors: Wen Et al.
PLoS One 2015;10:e0135591
Glucocorticoid regulates TrkB protein levels via c-Cbl dependent ubiquitination: a decrease in c-Cbl mRNA in the prefrontal cortex of suicide subjects.
Authors: Pandya Et al.
A critical role for protein degradation in the nucleus accumbens core in cocaine reward memory.
Authors: Ren Et al.
CDK5 is essential for soluble amyloid β-induced degradation of GKAP and remodeling of the synaptic actin cytoskeleton.
Authors: Roselli Et al.
PLoS One 2011;6:e23097
No product specific FAQs exist for this product, however you mayView all Small Molecule FAQs
Reviews for Lactacystin
Average Rating: 4 (Based on 1 Review)
Have you used Lactacystin?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
To tested the effects of inhibiting the proteolytic activity of the 26S proteasome complex via bath application of MG132 (50 M, 4 h) or lactacystin (10 M, 4 h) versus DMSO vehicle control.