LAIR1 Antibody (113) - BSA Free

Novus Biologicals | Catalog # NBP1-43324

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # 113

Format

BSA Free
View all LAIR1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD305.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Scientific Data Images for LAIR1 Antibody (113) - BSA Free

Flow Cytometry: LAIR1 Antibody (113) - BSA Free [NBP1-43324]

Flow Cytometry: LAIR1 Antibody (113) - BSA Free [NBP1-43324]

Flow Cytometry: LAIR1 Antibody (113) [NBP1-43324] - Staining of C57BL/6 splenocytes with 0.125 ug of Armenian Hamster IgG Isotype Control Purified (purple line) or 0.125 ug of Anti-Mouse CD305 (LAIR-1) Purified (blue line) followed by Anti-Armenian Hamster IgG PE. Total viable F4/80+ cells were used for analysis.

Applications for LAIR1 Antibody (113) - BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This 113 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LAIR1

CD305 or Leukocyte-associated Ig-like receptor-1 (LAIR-1) is a 46 kD cell surface molecule that functions as an inhibitory collagen-receptor on the majority of cells of the immune system. The mouse LAIR-1 molecule is expressed primarily on monocytes/macrophages and also on T cells, NK cells and dendritic cells. Unlike human B cells, mouse B cells do not express LAIR-1. The cytoplasmic tail of mouse LAIR-1 can be phosphorylated leading to the recruitment of SH2-containing tyrosine phosphatase(SHP)-2 and C-terminal Src kinase.

Long Name

Leukocyte-associated Ig-like Receptor 1

Alternate Names

CD305, HLAIR1, LAIR-1

Gene Symbol

LAIR1

Additional LAIR1 Products

Product Documents for LAIR1 Antibody (113) - BSA Free

Certificate of Analysis

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Product Specific Notices for LAIR1 Antibody (113) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LAIR1 Antibody (113) - BSA Free

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Protocols

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