Lambda Light Chain Antibody (LcN-2 + ICO-106)

Novus Biologicals | Catalog # NBP2-34236

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Kappa/IgG2a Kappa Clone # LcN-2 + ICO-106
View all Lambda Light Chain Primary Antibodies »

Product Specifications

Immunogen

Purified human Lambda Light Chain (LcN-2 and ICO-106)

Localization

Cell Surface, cytoplasmic and secreted

Marker

B-Cell Marker

Specificity

This monoclonal antibody is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Kappa/IgG2a Kappa

Theoretical MW

22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34665)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Lambda Light Chain Antibody (LcN-2 + ICO-106)

Western Blot: Lambda Light Chain Antibody (LcN-2 + ICO-106) [NBP2-34236]

Western Blot: Lambda Light Chain Antibody (LcN-2 + ICO-106) [NBP2-34236]

Western Blot: Lambda Light Chain Antibody (LcN-2 + ICO-106) [NBP2-34236] - Western blot analysis of Lambda Light Chain in human intestine using Lambda Light Chain Ab (LcN-2+ICO-106).
Immunocytochemistry/ Immunofluorescence: Lambda Light Chain Antibody (LcN-2 + ICO-106) [NBP2-34236]

Immunocytochemistry/ Immunofluorescence: Lambda Light Chain Antibody (LcN-2 + ICO-106) [NBP2-34236]

Immunocytochemistry/Immunofluorescence: Lambda Light Chain Antibody (LcN-2 + ICO-106) [NBP2-34236] - Staining of human LP-1 and H929 multiple myeloma cells. 1. Cells immersion-fixed in 2% PFA 10 minutes, wash PBS X 32. Permeabilized 0.5% Triton X-100 5 minutes; wash PBS X 33. Block 10% donkey serum 1 hour4. 1/200 NBP2-34236 O/N at 4C; wash PBS X 35. 1/1000 donkey F(ab')2 Alexa Fluor 647 anti-mouse IgG (H+L) 1 hour at room temperature and wash with PBS X 3.6. Olympus/BIOVIEW Cy5 filter 600ms exposure. Image from a verified customer review.

Applications for Lambda Light Chain Antibody (LcN-2 + ICO-106)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-34236 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Lambda Light Chain

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30, the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.

Alternate Names

IGLC, immunoglobulin lambda constant group

Gene Symbol

IGLC1

UniProt

P01701 (Human), P01842 (Human)

Additional Lambda Light Chain Products

Product Documents for Lambda Light Chain Antibody (LcN-2 + ICO-106)

Certificate of Analysis

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Product Specific Notices for Lambda Light Chain Antibody (LcN-2 + ICO-106)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Lambda Light Chain Antibody (LcN-2 + ICO-106) (1)

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  • Lambda Light Chain Antibody (LcN-2 + ICO-106)
    Name: Natalie Erdmann
    Application: Immunocytochemistry
    Sample Tested: LP-1 (multiple myeloma) and H929 (multiple myeloma)
    Species: Human
    Verified Customer | Posted 06/17/2019
    Immunocytochemistry/immunofluorescence staining of LP-1 and H929 multiple myeloma cells with NBP2-34236 Lambda light chain antibody
    1. Cells immersion-fixed in 2%PFA 10 minutes, wash PBS X 3 2. Permeabilized 0.5% Triton X-100 5 minutes; wash PBS X 3 3. Block 10% donkey serum 1 hour 4. 1/200 NBP2-34236 O/N @4oC; wash PBS X 3 5. 1/1000 donkey F(ab’)2 Alexa Fluor 647 anti-mouse IgG (H+L) 1 hour @room temp (Jackson #715-606-150); wash PBS X 3 6. Olympus/BIOVIEW Cy5 filter 600ms exposure
    Lambda Light Chain Antibody (LcN-2 + ICO-106) NBP2-34236

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